Anti-IL-6R 抗体 [AP-MAB0848] (ab83053)
Key features and details
- Rat monoclonal [AP-MAB0848] to IL-6R
- Suitable for: Inhibition Assay, IP, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse
- Isotype: IgG2b
製品の概要
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製品名
Anti-IL-6R antibody [AP-MAB0848]
IL-6R 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [AP-MAB0848] to IL-6R -
由来種
Rat -
アプリケーション
適用あり: Inhibition Assay, IP, ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant full length protein corresponding to Mouse IL-6R.
Database link: P22272 -
特記事項
This monoclonal can be used to block IL-6/IL-6R interactions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
The IgG fraction of culture supernatant was purified by Protein A/G affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate buffered saline. -
一次抗体 備考
This monoclonal can be used to block IL-6/IL-6R interactions. -
ポリ/モノ
モノクローナル -
クローン名
AP-MAB0848 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83053の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Inhibition Assay |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa.
|
| 特記事項 |
|---|
|
Inhibition Assay
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 52 kDa. |
ターゲット情報
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機能
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
組織特異性
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
配列類似性
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
翻訳後修飾
A short soluble form may also be released from the membrane by proteolysis. -
細胞内局在
Secreted and Basolateral cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16194 Mouse
- SwissProt: P22272 Mouse
- Unigene: 2856 Mouse
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別名
- CD 126 antibody
- CD126 antibody
- CD126 antigen antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab83053 は 13 報の論文で使用されています。
- Zhou T et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv 8:eabj9617 (2022). PubMed: 35020440
- Dong J et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
- Rupert JE et al. Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia. J Exp Med 218:N/A (2021). PubMed: 33851955
- Cui H & Zhang Q Dexmedetomidine ameliorates lipopolysaccharide-induced acute lung injury by inhibiting the PI3K/Akt/FoxO1 signaling pathway. J Anesth 35:394-404 (2021). PubMed: 33821300
- Alves-Silva T et al. Interleukin-6 deficiency modulates testicular function by increasing the expression of suppressor of cytokine signaling 3 (SOCS3) in mice. Sci Rep 11:11456 (2021). PubMed: 34075113