Anti-IL-6 抗体 [EPR23819-103] (ab290735)

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Immunohistochemistry (Frozen sections) - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23819-103] to IL-6
Suitable for: ICC/IF, IHC-P, WB, IHC-Fr
Reacts with: Mouse

Carrier Free

製品名

Anti-IL-6 antibody [EPR23819-103]
IL-6 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR23819-103] to IL-6
由来種

Rabbit
アプリケーション

適用あり: ICC/IF, IHC-P, WB, IHC-Frmore details
適用なし: Flow Cyt (Intra) or IP
種交差性

交差種: Mouse
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate and RAW264.7 treated with 0.1µg/ml lipopolysaccharide (LPS) for 4 hours, then with 1 µg/ml Brefeldin A (BFA) added for 3 hours, whole cell lysate. IHC-P: Mouse spleen and lung tissue, HEK-293T cells transfected with mouse IL-6 expression vector containing a his tag. ICC/IF: treated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage). IHC-Fr : Mouse spleen tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR23819-103
アイソタイプ

IgG
研究分野
- Neuroscience
- Processes

Compatible Secondaries
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
Related Products
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab290735の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
ICC/IF		1/50.
IHC-P		1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa). Compared with ab290735, ab229381 and ab259341 have higher affinity. We recommend ab229381 or ab259341 as alternatives for western blot.
IHC-Fr		1/50.

特記事項
ICC/IF 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa). Compared with ab290735, ab229381 and ab259341 have higher affinity. We recommend ab229381 or ab259341 as alternatives for western blot.
IHC-Fr 1/50.

追加情報

Is unsuitable for Flow Cyt (Intra) or IP.

機能

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
関連疾患

Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
配列類似性

Belongs to the IL-6 superfamily.
翻訳後修飾

N- and O-glycosylated.
細胞内局在

Secreted.
Target information above from: UniProt accession P05231 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 16193 Mouse
- SwissProt: P08505 Mouse
- Unigene: 1019 Mouse
別名
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
- B-cell stimulatory factor 2 antibody
- BSF 2 antibody
- BSF-2 antibody
- BSF2 antibody
- CDF antibody
- CTL differentiation factor antibody
- Cytotoxic T cell differentiation factor antibody
- Hepatocyte stimulating factor antibody
- Hepatocyte stimulatory factor antibody
- HGF antibody
- HSF antibody
- Hybridoma growth factor antibody
- Hybridoma growth factor Interferon beta-2 antibody
- Hybridoma plasmacytoma growth factor antibody
- IFN-beta-2 antibody
- IFNB2 antibody
- IL 6 antibody
- IL-6 antibody
- IL6 antibody
- IL6_HUMAN antibody
- Interferon beta 2 antibody
- Interferon beta-2 antibody
- Interleukin 6 antibody
- Interleukin 6 (interferon beta 2) antibody
- Interleukin BSF 2 antibody
- Interleukin-6 antibody
see all

Immunohistochemistry (Frozen sections) - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh mouse spleen tissue labeling IL-6 with ab290735 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed secondary at 1/1000. Increased staining on mouse spleen treated with lipopolysaccharide (1 μg/mL) and Brefeldin A (1 μg/mL) for 16 h. The nuclear counterstain was DAPI (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Immunohistochemical analysis of paraffin-embedded Mouse spleen treated tissue labelling IL-6 with ab290735 at 1/100 (5.75 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection)

Positive staining on mouse spleen treated with lipopolysaccharides (1 ug/ml) for 16 h in vitro (A) and no staining on untreated mouse spleen (B). The section was incubated with ab290735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Immunohistochemical analysis of paraffin-embedded Mouse lung treated tissue labelling IL-6 with ab290735 at 1/100 (5.75 ug/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on Mouse lung treated with lipopolysaccharides (1 ug/ml) for 16 h in vitro (A) and no staining on untreated mouse lung (B). The section was incubated with ab290735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with mouse IL-6 expression vector containing a his tag, labelling IL-6 with ab290735 at 1/100 (5.75 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on HEK-293T cells transfected with mouse IL-6 expression vector containing a his tag (A). No staining on HEK-293T cells transfected with empty vector containing a his tag (B). The section was incubated with ab290735 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins
Western blot - Anti-IL-6 antibody [EPR23819-103] (ab290735)

All lanes : Anti-IL-6 antibody [EPR23819-103] (ab290735) at 1/1000 dilution

Lanes 1 & 3 & 5 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lanes 2 & 4 & 6 : RAW264.7 treated with 0.1µg/ml lipopolysaccharide (LPS) for 4 hours, then with 1 µg/ml Brefeldin A (BFA) added for 3 hours, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-2 : Anti-IL-6 antibody [EPR23819-103] (ab290735) at 1/1000 dilution
Lanes 3-4 : Anti-IL-6 antibody [EPR21710] (ab229381) at 1/1000 dilution
Lanes 5-6 : Anti-IL-6 antibody [EPR23819-11] (ab259341) at 1/1000 dilution

The expression profile/molecular weight observed is consistent with that described in the literature (PMID:2523818, PMID:25130514).
IL-6 expressed in LPS-induced RAW264.7 cells (PMID:31718640, PMID:30823686).

Compared with ab290735, ab229381 and ab259341 have higher affinity. We recommend ab229381 or ab259341 as alternatives for western blot.
Western blot - Anti-IL-6 antibody [EPR23819-103] (ab290735)

All lanes : Anti-IL-6 antibody [EPR23819-103] (ab290735) at 1/1000 dilution

Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate.
Lane 2 : RAW264.7 treated with 0.1µg/ml lipopolysaccharide (LPS) for 4 hours, then with 1 µg/ml Brefeldin A (BFA) added for 3 hours whole cell lysate.

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20 dilution

Predicted band size: 23 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking / Dilution buffer and concentration: 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with that described in the literature (PMID:2523818, PMID:25130514).
IL6 expressed in LPS-induced RAW264.7 cells (PMID:31718640, PMID:30823686).
Immunocytochemistry/ Immunofluorescence - Anti-IL-6 antibody [EPR23819-103] (ab290735)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells lebelling IL-6 with ab290735 at 1/50 (11.5 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/ml dilution) (Green). Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with LPS (100 ng/ml) for 4h, with addition of BFA (1 ug/ml) for the last 3 hours is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2ug/ml) dilution.
Anti-IL-6 antibody [EPR23819-103] (ab290735)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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ab290735 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab290735 は 11 報の論文で使用されています。

Peng K et al. Rosmarinic acid protects against lipopolysaccharide-induced cardiac dysfunction via activating Sirt1/PGC-1α pathway to alleviate mitochondrial impairment. Clin Exp Pharmacol Physiol 50:218-227 (2023). PubMed: 36350269
Varlamova EG et al. Antifibrotic Effect of Selenium-Containing Nanoparticles on a Model of TAA-Induced Liver Fibrosis. Cells 12:N/A (2023). PubMed: 38067151
Ding Z et al. Nanosized Silk-Magnesium Complexes for Tissue Regeneration. Adv Healthc Mater 12:e2300887 (2023). PubMed: 37317936
Zhou S et al. Parishin treatment alleviates cardiac aging in naturally aged mice. Heliyon 9:e22970 (2023). PubMed: 38144278
Youjun D et al. Mechanisms of resveratrol against diabetic wound by network pharmacology and experimental validation. Ann Med 55:2280811 (2023). PubMed: 37967241

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Mammary tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA citrate(pH 9.0)

Specification

Mammary tumor

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 27°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 22 2023

Application

Immunocytochemistry/ Immunofluorescence

Sample

Syrian hamster Cell (lung tissue)

Permeabilization

Yes - 1% Triton X-100 in PBS

Specification

lung tissue

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 28 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (11)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-6 antibody [EPR23819-103]

Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-6 antibody [EPR23819-103]