Anti-IL-6 抗体 [1.2-2B11-2G10] (ab9324)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.co.jp/ps/products/9/ab9324/Images/ab9324-454107-anti-il-6-antibody-immunohistochemistry-liver-tumor-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)")
Key features and details
- Mouse monoclonal [1.2-2B11-2G10] to IL-6
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-IL-6 antibody [1.2-2B11-2G10]
IL-6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1.2-2B11-2G10] to IL-6 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, Sandwich ELISA, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
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ポジティブ･コントロール
- IHC-P: Human liver tumor and prostate tumor tissues; WB: Human spleen and lung tissue lysates, Rat spleen and lung tissue lysates, Recombinant human IL6 protein (ab9627).
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特記事項
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
1.2-2B11-2G10 -
アイソタイプ
IgG2a -
研究分野
関連製品
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9324の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (11) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Sandwich ELISA |
Use at an assay dependent concentration.
In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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| WB | (3) |
Use at an assay dependent concentration.
To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
| 特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Sandwich ELISA
Use at an assay dependent concentration. In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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WB
Use at an assay dependent concentration. To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
ターゲット情報
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機能
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
関連疾患
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
配列類似性
Belongs to the IL-6 superfamily. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3569 Human
- Entrez Gene: 24498 Rat
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P20607 Rat
- Unigene: 654458 Human
- Unigene: 9873 Rat
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別名
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.co.jp/ps/products/9/ab9324/Images/ab9324-251202-anti-il-6-antibody-western-blot.jpg)
Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)All lanes : Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) at 1/2000 dilution
Lane 1 : Human Spleen
Lane 2 : Human Lung
Lane 3 : Rat Spleen
Lane 4 : Rat Lung
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : 800CW Goat Anti-Mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17,25 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible multimer)This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.co.jp/ps/products/9/ab9324/Images/ab9324-454110-anti-il-6-antibody-immunohistochemistry-prostate-tumor-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.co.jp/ps/products/9/ab9324/Images/ab9324-400102-Western-blot--AntiIL6-antibody-ab9324.png)
Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)ab9324 detecting IL-6 by sandwich ELISA.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (265)
ab9324 は 265 報の論文で使用されています。
- Ye T et al. Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity. Bioact Mater 21:194-208 (2023). PubMed: 36101856
- Wang XK et al. TNAP-a potential cytokine in the cerebral inflammation in spastic cerebral palsy. Front Mol Neurosci 15:926791 (2022). PubMed: 36187348
- Chen Y et al. Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells. Front Neurosci 16:990040 (2022). PubMed: 36188472
- Liu L et al. Hepatitis B virus promotes hepatocellular carcinoma development by activating GP73 to repress the innate immune response. Infect Agent Cancer 17:52 (2022). PubMed: 36195933
- Tang D et al. Network pharmacology-based prediction of active compounds in the Wenyang Jiedu Huayu formula acting on acute-on-chronic liver failure with experimental support in vitro and in vivo. Front Pharmacol 13:1003479 (2022). PubMed: 36339606