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AB324011

Anti-IL-5RA 抗体 [EPR28313-257]

Anti-IL-5RA antibody [EPR28313-257]

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Rabbit Recombinant Monoclonal IL-5RA antibody. Suitable for Flow Cyt and reacts with Human, Mouse samples.

別名を表示する

CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125

3 Images
Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)

Flow cytometric analysis of Human purified neutrophils cells labelling IL-5RA with ab324011 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)

Flow cytometric analysis of Fresh mouse blood cells labelling IL-5RA with ab324011 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Fresh mouse blood cells were co-stained with CD11b conjugated to Brilliant Violet 421.
Gated on viable granulocyte population(SSC-A high).

Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)

Flow cytometric analysis of Fresh mouse blood cells labelling IL-5RA with ab324011 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Fresh mouse blood cells were co-stained with CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR28313-257

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Mouse

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB283549

Human IL-5 Receptor alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
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Primary Antibodies

AB207737

Anti-IL-33 antibody [EPR20417]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR20417] (AB207737)

  • 0
  • 0
Assay Kits

AB283549

Human IL-5 Receptor alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-5 Receptor alpha ELISA Kit (AB283549)

  • 0
  • 0
Primary Antibodies

AB133575

Anti-IL-10 antibody [EPR1114]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IL-10 antibody [EPR1114] (AB133575)

  • 4
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed : 1495999, PubMed : 22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed : 9516124).
See full target information IL5RA
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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