Anti-IL-4 抗体 (ab9622)
Key features and details
- Rabbit polyclonal to IL-4
- Suitable for: ELISA, WB, Sandwich ELISA, Neutralising
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-4 antibody
IL-4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-4 -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, Sandwich ELISA, Neutralisingmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human IL-4. Highly pure (>98%) recombinant hIL-4 (human Interleukin-4)
Database link: P05112 -
ポジティブ･コントロール
- Recombinant human IL4 protein (ab9623) can be used as a positive control in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
No preservative, sterile filtered -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
To detect hIL-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
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| Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as Detection antibody with recommended pair. |
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| Neutralising |
Use at an assay dependent concentration.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. |
| 特記事項 |
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ELISA
Use at an assay dependent concentration. To detect hIL-4 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 15 kDa. To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa. |
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Sandwich ELISA
Use at an assay dependent concentration. Can be used as Detection antibody with recommended pair. |
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Neutralising
Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. |
ターゲット情報
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機能
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. -
関連疾患
Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
配列類似性
Belongs to the IL-4/IL-13 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3565 Human
- Omim: 147780 Human
- SwissProt: P05112 Human
- Unigene: 73917 Human
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別名
- B cell growth factor 1 antibody
- B cell IgG differentiation factor antibody
- B Cell Stimulatory Factor 1 antibody
see all
画像
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Western blot - Anti-IL-4 antibody (ab9622)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab9622 は 35 報の論文で使用されています。
- Virgens AR et al. Perivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinone. Exp Dermatol 31:191-201 (2022). PubMed: 34358352
- Kurkipuro J et al. Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds. PLoS One 17:e0264775 (2022). PubMed: 35226700
- Toney NJ et al. Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers. J Transl Med 20:112 (2022). PubMed: 35255925
- Shukla M et al. Moderate noise associated oxidative stress with concomitant memory impairment, neuro-inflammation and neurodegeneration. Brain Behav Immun Health 5:100089 (2020). PubMed: 34589861
- Baba T et al. Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration. Elife 9:N/A (2020). PubMed: 32366355