Anti-IL-34 抗体 (ab74548)
Key features and details
- Rabbit polyclonal to IL-34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-34 antibody
IL-34 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-34 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IL-34 (N terminal). (ACD13474)
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ポジティブ･コントロール
- Human brain tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab74548の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.25 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 27 kDa). |
ターゲット情報
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機能
Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R. -
組織特異性
Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon. -
配列類似性
Belongs to the IL-34 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 146433 Human
- Omim: 612081 Human
- SwissProt: Q6ZMJ4 Human
- Unigene: 461214 Human
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別名
- C16orf77 antibody
- Chromosome 16 open reading frame 77 antibody
- FPT025 antibody
see all
画像
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Western blot - Anti-IL-34 antibody (ab74548)Anti-IL-34 antibody (ab74548) at 1 µg/ml + Recombinant human IL-34 protein at 0.001 µg
Predicted band size: 27 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Western blot - Anti-IL-34 antibody (ab74548)All lanes : Anti-IL-34 antibody (ab74548) at 0.25 µg/ml
Lane 1 : Human brain tissue lysate, minus immunising peptide
Lane 2 : Human brain tissue lysate, plus immmunising peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 27 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab74548 は 1 報の論文で使用されています。
- Walker DG et al. Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia. Front Aging Neurosci 9:244 (2017). PubMed: 28848420