Name: Anti-IL-33 antibody [EPR17831]
Brand: RabMAb
SKU: ab187060
Rating: 5 (2 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17831] to IL-33
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat

Alexa Fluor® 488 APC Carrier Free PE

製品名

Anti-IL-33 antibody [EPR17831]
IL-33 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR17831] to IL-33
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP
種交差性

交差種: Mouse, Rat
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Rat and mouse lung tissue lysate; RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate. IHC-P: Mouse and rat spleen tissue. ICC/IF: RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours cells. Flow Cyt (intra): RAW 264.7 cells
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR17831
アイソタイプ

IgG
研究分野

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab187060の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/500.
WB		1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 30 kDa).
IHC-P	(1)	1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
ICC/IF	(1)	1/500.

特記事項
Flow Cyt (Intra) 1/500.
WB 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 30 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
ICC/IF 1/500.

追加情報

Is unsuitable for IP.

機能

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
組織特異性

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
配列類似性

Belongs to the IL-1 family. Highly divergent.
ドメイン

The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
翻訳後修飾

The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
細胞内局在

Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
Target information above from: UniProt accession O95760 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 77125 Mouse
- Entrez Gene: 361749 Rat
- SwissProt: Q8BVZ5 Mouse
- SwissProt: Q66H70 Rat
別名
- C9orf26 antibody
- CHROMOSOME 9 OPEN READING FRAME 26 antibody
- DKFZp586H0523 antibody
- DVS27 antibody
- DVS27 related protein antibody
- IL 1F11 antibody
- IL 33 antibody
- IL-1F11 antibody
- IL-33 antibody
- IL1F11 antibody
- IL33 antibody
- IL33_HUMAN antibody
- Interleukin 1 family member 11 antibody
- Interleukin 33 antibody
- INTERLEUKIN 33 NFHEV antibody
- Interleukin 33 precursor antibody
- Interleukin-1 family member 11 antibody
- Interleukin-33 (109-270) antibody
- Interleukin33 antibody
- NF HEV antibody
- NF-HEV antibody
- NFEHEV antibody
- NFHEV antibody
- Nuclear factor for high endothelial venules antibody
- Nuclear factor from high endothelial venules antibody
- OTTHUMP00000021041 antibody
- RP11 575C20.2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunohistochemical analysis of paraffin-embedded (A) Lung tissue from wild-type C57BL/6JGpt mice (B) Lung tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/5000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Lung tissue from wild-type C57BL/6JGpt mice and no staining on (B) Lung tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunohistochemical analysis of paraffin-embedded (A) Lymph node tissue from wild-type C57BL/6JGpt mice (B) Lymph node tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/5000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Lymph node tissue from wild-type C57BL/6JGpt mice and no staining on (B) Lymph node tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice (B) Spleen tissue from IL-33 knockout mice labeling IL33 with ab187060 at 1/2000 dilution, followed by LeicaDS9800 (Bond™ Polymer Refine Detection), ready to use. Counter stained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from IL-33 knockout mice.
The section was incubated with ab187060 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)

All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution

Lane 1 : Wild-type mouse spleen tissue lysate (male)
Lanes 2-3 : IL-33 knockout spleen tissue lysate (male)
Lane 4 : Wild-type mouse inguinal lymph nodes tissue lysate (male)
Lanes 5-6 : IL-33 knockout inguinal lymph nodes tissue lysate (male)
Lane 7 : Wild-type mouse submandibular lymph nodes tissue lysate (male)
Lanes 8-9 : IL-33 knockout submandibular lymph nodes tissue lysate (male)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 15-30 kDa why is the actual band size different from the predicted?

Exposure time: 38 seconds

Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa)

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)

All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution

Lane 1 : Wild-type mouse lung tissue lysate (male)
Lanes 2-3 : IL-33 knockout lung tissue lysate (male)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 15-30 kDa why is the actual band size different from the predicted?

Exposure time: 38 seconds

Loading control: Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1/200000) (36KDa)

Blocking/Diluting buffer and concentration: 5% NFDM/TBST

The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and IL33-KO homozygous mice (Strain ID: T052437).
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)

Flow cytometry overlay histogram showing left, Raw264.7 treated with 50nM PMA and 5μg/ml LPS for 24h and right, negative untreated Raw264.7 stained with ab187060 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab187060) (1x 10⁶ in 100μl at 1.0μg/ml (1/2090)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody (black line) was Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)

All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution

Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Developed using the ECL technique.

Predicted band size: 30 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST

Exposure: 3 minutes

IL-33 expression is induced by LPS treatment of PMA-differentiated RAW 264.7 cells (PMID 19559631; PMID 19933859).
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)

All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution

Lane 1 : Rat lung tissue lysate at 20 µg
Lane 2 : Mouse lung tissue lysate at 10 µg

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 30 kDa
Observed band size: 30 kDa

Blocking/Dilution: 5% NFDM/TBST

Exposure: 3 minutes
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)

Intracellular Flow Cytometry analysis ofRAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50nM PMA and 5µg/ml LPS for 24h (Red) / Untreated control (Green) labeling IL-33 with ab187060 at 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody. Cells were fixed with4% paraformaldehyde and permeabilised with0.1% Tween-20.Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in endothelial cells of mouse spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in endothelial cells of rat spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (ab187060)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling IL33 with ab187060 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining in RAW 264.7 cells treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml Lipopolysaccharide for 24h.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-IL-33 antibody [EPR17831] (ab187060)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab187060 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187060 は 18 報の論文で使用されています。

Song J et al. Bifidobacterium mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway. Histol Histopathol 39:623-632 (2024). PubMed: 37916940
Varlamova EG et al. Antifibrotic Effect of Selenium-Containing Nanoparticles on a Model of TAA-Induced Liver Fibrosis. Cells 12:N/A (2023). PubMed: 38067151
Sun P et al. Decellularized fish swim bladder patch loaded with mesenchymal stem cells inhibits neointimal hyperplasia. J Biomed Mater Res B Appl Biomater 111:551-559 (2023). PubMed: 36200602
Van Mechelen M et al. Impact of barrier tissue inflammation and physical activity on joint homeostasis in mice. Rheumatology (Oxford) 61:1690-1698 (2022). PubMed: 34175921
Wang H et al. IL-33 Promotes ST2-Dependent Fibroblast Maturation via P38 and TGF-β in a Mouse Model of Epidural Fibrosis. Tissue Eng Regen Med 19:577-588 (2022). PubMed: 35195855

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer, PH=6

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Fixative

10% Formalin

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Hongyan Zhao

Verified customer

投稿 Sep 09 2022

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Lymph node sections)

Permeabilization

Specification

Lymph node sections

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Nagham Alouche

Verified customer

投稿 Apr 19 2021

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (18)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-33 antibody [EPR17831]

Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-33 antibody [EPR17831]