Anti-IL-31 抗体 (ab62579)
Key features and details
- Rabbit polyclonal to IL-31
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-31 antibody
IL-31 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-31 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IL-31 (internal sequence).
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ポジティブ･コントロール
- Human skeletal muscle tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62579の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 20 µg/ml.
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| WB | (1) |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE. |
ターゲット情報
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機能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
組織特異性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 386653 Human
- Omim: 609509 Human
- SwissProt: Q6EBC2 Human
- Unigene: 569071 Human
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製品の状態
Amino acids 1-23 are a signal peptide. -
別名
- IL 31 antibody
- IL-31 antibody
- IL31 antibody
see all
画像
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Western blot - Anti-IL-31 antibody (ab62579)Lane 1 : Anti-IL-31 antibody (ab62579) at 2.5 µg/ml
Lane 2 : Anti-IL-31 antibody (ab62579) at 5 µg/ml
Lane 3 : Anti-IL-31 antibody (ab62579) at 10 µg/ml
All lanes : human skeletal muscle tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-31 antibody (ab62579)Immunofluorescence of IL-31 in Human Skeletal Muscle cells using ab62579 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62579 は論文での使用が確認できていません。