Anti-IL-3 抗体 [EPR7964] (ab167159)
![Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)](https://www.abcam.co.jp/ps/products/167/ab167159/Images/ab167159-3-anti-il3-antibody-epr7964-western-blot-mouse.jpg "Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7964] to IL-3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-3 antibody [EPR7964]
IL-3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7964] to IL-3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human IL-3. The exact sequence is proprietary.
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ポジティブ･コントロール
- Jurkat cells. Human recombinant IL3 protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR7964 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab167159の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
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| ICC/IF |
1/100 - 1/250.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa. |
|
ICC/IF
1/100 - 1/250. |
追加情報
Is unsuitable for IHC-P or IP.
ターゲット情報
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機能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. -
組織特異性
Activated T-cells, mast cells, natural killer cells. -
配列類似性
Belongs to the IL-3 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3562 Human
- Entrez Gene: 16187 Mouse
- Omim: 147740 Human
- SwissProt: P08700 Human
- SwissProt: P01586 Mouse
- Unigene: 694 Human
- Unigene: 983 Mouse
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別名
- Colony stimulating factor multiple antibody
- Hematopoietic growth factor antibody
- IL 3 antibody
see all
画像
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Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)Anti-IL-3 antibody [EPR7964] (ab167159) at 1.23 µg/ml + Mouse IL-3 recombinant protein at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 17 kDa
Observed band size: 17 kDa
Blocking/diluting Buffer and concentration: 5% NFDM /TBST -
![Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (ab167159)](https://www.abcam.co.jp/ps/products/167/ab167159/Images/ab167159-165475-anti-il-3-antibody-epr7964-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-3 antibody [EPR7964] (ab167159)Immunocytochemical analysis of Jurkat cells labeling IL3 with ab167159 at a 1/100 dilution. -
![Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)](https://www.abcam.co.jp/ps/products/167/ab167159/Images/ab167159-165476-anti-il-3-antibody-epr7964-western-blot.jpg)
Western blot - Anti-IL-3 antibody [EPR7964] (ab167159)Anti-IL-3 antibody [EPR7964] (ab167159) at 1/1000 dilution + Human IL3 recombinant protein (10 ng)
Predicted band size: 17 kDa -
![Anti-IL-3 antibody [EPR7964] (ab167159)](https://www.abcam.co.jp/ps/products/167/ab167159/Images/ab167159-4-benefits-of-recombinant-antibodies.png)
Anti-IL-3 antibody [EPR7964] (ab167159)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab167159 は 1 報の論文で使用されています。
- Zhao DD et al. Evidence for a role of geranylgeranylation in renal angiomyolipoma and renal epithelioid angiomyolipoma. Oncol Lett 17:1523-1530 (2019). PubMed: 30675208