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Anti-IL-25 抗体 [68C1039.2] (ab115672)

Reviews (1)Q&A (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-25 antibody [68C1039.2] (ab115672)

    Key features and details

    • Mouse monoclonal [68C1039.2] to IL-25
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-IL-25 antibody [68C1039.2]
      IL-25 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [68C1039.2] to IL-25

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Pig

    • 免疫原

      Synthetic peptide corresponding to Human IL-25 aa 100-200 conjugated to keyhole limpet haemocyanin.
      Database link: NP_073626.1

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human Adrenal Cortex tissue.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at -20ºC.

    • バッファー

      Preservative: 0.05% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...

    • 精製度

      Protein G purified

    • ポリ/モノ

      モノクローナル

    • クローン名

      68C1039.2

    • アイソタイプ

      IgG1

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab115672の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses.

    • 組織特異性

      Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea.

    • 配列類似性

      Belongs to the IL-17 family.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • IL 17E antibody
        • IL 25 antibody
        • IL-17E antibody
        • IL-25 antibody
        • IL17e antibody
        • IL25 antibody
        • IL25_HUMAN antibody
        • Interleukin 17E antibody
        • Interleukin 25 antibody
        • Interleukin-17E antibody
        • Interleukin-25 antibody
        • Interleukin17E antibody
        • Interleukin25 antibody
        • OTTHUMP00000027946 antibody
        • OTTHUMP00000246238 antibody
        • UNQ3120/PRO10272 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-25 antibody [68C1039.2] (ab115672)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-25 antibody [68C1039.2] (ab115672)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal cortex tissue, staining IL17E with ab115672 at 10 µg/ml.

      参考文献 (0)

      ab115672 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab115672 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-IL-17E antibody [68C1039.2]

       Inconclusive
      Abreviews
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Sheep Tissue sections (Lung tissue and lymph node)
      Permeabilization
      No
      Specification
      Lung tissue and lymph node
      Blocking step
      (agent) for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Acetone
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 25 2012

      Question

      We are considering purchasing 2 further antibodies from Abcam to the one that I will use from the previously generated discount. As such, these will not be bought using the discount code that was previously generated for the EGFR antibody ab2430.

      Antibody ab106732 (IL-13) has not been listed as working in sheep. We would like to test the antibody for IHC-Fr and I was wondering whether we would be able to get the 100% testing discount for this antibody.

      The second antibody is the IL-25 antibody (ab115672) and will also be used in IHC-Fr on sheep tissue. In this instance sheep is listed under the predicted to react with tab. Would this also qualify for the testing discount? Or does it fall under your guarantee?

      Thanks in advance for your help.


      Kind Regards

      Read More
      Answer

      DISCOUNT CODEs

      ab106732 : 0000

      ab115672 : 00000

      Expiration date: 16. November 2012

      I can confirm that the discount codescan be redeemed with Sapphire Biosciences. Predicted species are not covered by the Abpromise and therefore I am happy to provide a testing discount.

      I am very pleased to hear you would like to accept our offer and testthese above mentioned antibodiesin sheep.Each code will give you the value of the tested antibody off a future order before the expiration date. To redeem this offer, please submit an Abreview for sheep and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: ˜
      1) Call to place your order and mention the code to our customer service department;
      2) Include the code in your fax order;
      3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com