Anti-IL-24 抗体 (ab115207)
Key features and details
- Rabbit polyclonal to IL-24
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-24 antibody
IL-24 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-24 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IL-24 aa 100-200 conjugated to keyhole limpet haemocyanin.
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ポジティブ･コントロール
- WB: Jurkat cell lysate. IHC-P: Human prostate tissue. Flow Cyt (Intra): Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.2% Gelatin, 99% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115207の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 24 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. After incubation with the primary antibody, slides are incubated with biotinylated rabbit anti-goat IgG secondary antibody followed by alkaline phosphatase-streptavidin and chromogen.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 24 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. After incubation with the primary antibody, slides are incubated with biotinylated rabbit anti-goat IgG secondary antibody followed by alkaline phosphatase-streptavidin and chromogen. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation. -
組織特異性
Up-regulated in melanoma cells induced to terminally differentiate. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 11009 Human
- Omim: 604136 Human
- SwissProt: Q13007 Human
- Unigene: 58831 Human
- Unigene: 728324 Human
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別名
- C49A antibody
- FISP antibody
- IL 24 antibody
see all
画像
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Western blot - Anti-IL-24 antibody (ab115207)All lanes : Anti-IL-24 antibody (ab115207) at 0.5 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat cell lysate with IL24 blocking peptide
Predicted band size: 24 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-24 antibody (ab115207)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, staining IL24 with ab114207 at 10 µg/ml. -
Flow Cytometry (Intracellular) - Anti-IL-24 antibody (ab115207)Fow cytometry analysis of IL24 in Jurkat cells using ab115207at 5µg/106 cells. The shaded histogram represents cells only, green represents isotype control, and pink represents anti-IL24 antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (4)
ab115207 は 4 報の論文で使用されています。
- Chang SC et al. Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment. Int J Mol Sci 22:N/A (2021). PubMed: 33562773
- Wu Z et al. Mesenchymal stem cells derived from iPSCs expressing interleukin-24 inhibit the growth of melanoma in the tumor-bearing mouse model. Cancer Cell Int 20:33 (2020). PubMed: 32015693
- Yang J et al. Tumor-Penetrating Peptide Enhances Antitumor Effects of IL-24 Against Prostate Cancer. Transl Oncol 12:453-461 (2018). PubMed: 30580153
- Margaryan NV et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci 17:418 (2016). WB . PubMed: 27011171
