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AB190356

Anti-IL-23 抗体 [EPR5585(N)] - C-terminal

Anti-IL-23 antibody [EPR5585(N)] - C-terminal

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(6 Publications)

Rabbit Recombinant Monoclonal IL-23 antibody. C-terminal. Suitable for WB and reacts with Recombinant full length protein, Human samples. Cited in 6 publications.

別名を表示する

SGRF, UNQ2498/PRO5798, IL23A, Interleukin-23 subunit alpha, IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19

2 Images
Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (AB190356)
  • WB

Supplier Data

Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (AB190356)

All lanes:

Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/1000 dilution

Lane 1:

THP-1 treated with GM-CSF+IL-4 cell lysate at 10 µg

Lane 2:

Untreated THP-1 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 21 kDa

false

Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (AB190356)
  • WB

Supplier Data

Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (AB190356)

All lanes:

Western blot - Anti-IL-23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/200 dilution

All lanes:

IL23 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 113 kDa,20 kDa

Observed band size: 65 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-IL-23 antibody [EPR5585(N)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5585(N)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed : 11114383). Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed : 29287995, PubMed : 32474165, PubMed : 33606986). This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A (PubMed : 12023369). In turn, participates in the early and effective intracellular bacterial clearance (PubMed : 32474165). Promotes the expansion and survival of T-helper 17 cells, a CD4-positive helper T-cell subset that produces IL-17, as well as other IL-17-producing cells (PubMed : 17676044).
See full target information IL23A
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 29: PubMed38516774

2024

miRNA‑21 promotes the progression of acute liver failure via the KLF6/autophagy/IL‑23 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Suxia Bao,Weiyang Zheng,Rong Yan,Jie Xu

International journal of experimental pathology 105:21-32 PubMed38054576

2023

Circular RNA circVAPA modulates macrophage pyroptosis in sepsis-induced acute lung injury through targeting miR-212-3p/Sirt1/Nrf2/NLRP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjing Huang,Jinquan Lin,Zhiwei Wu,Yiming Li

Nature communications 13:6782 PubMed36351945

2022

Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M Sodir,Luca Pellegrinet,Roderik M Kortlever,Tania Campos,Yong-Won Kwon,Shinseog Kim,Daniel Garcia,Alessandra Perfetto,Panayiotis Anastasiou,Lamorna Brown Swigart,Mark J Arends,Trevor D Littlewood,Gerard I Evan

Asian journal of andrology 24:147-153 PubMed34755700

2021

High IL-23+ cells infiltration correlates with worse clinical outcomes and abiraterone effectiveness in patients with prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Liu,Jun-Yu Zhang,Yun-Jie Yang,Kun Chang,Qi-Feng Wang,Yun-Yi Kong,Bo Dai

International journal of molecular medicine 42:2903-2913 PubMed30132508

2018

Inhibition of lncRNA NEAT1 suppresses the inflammatory response in IBD by modulating the intestinal epithelial barrier and by exosome-mediated polarization of macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Liu,Anliu Tang,Xiaoyan Wang,Xiong Chen,Lian Zhao,Zhiming Xiao,Shourong Shen

Nature 559:363-369 PubMed29950727

2018

IL-23 secreted by myeloid cells drives castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Calcinotto,Clarissa Spataro,Elena Zagato,Diletta Di Mitri,Veronica Gil,Mateus Crespo,Gaston De Bernardis,Marco Losa,Michela Mirenda,Emiliano Pasquini,Andrea Rinaldi,Semini Sumanasuriya,Maryou B Lambros,Antje Neeb,Roberta Lucianò,Carlo A Bravi,Daniel Nava-Rodrigues,David Dolling,Tommaso Prayer-Galetti,Ana Ferreira,Alberto Briganti,Antonio Esposito,Simon Barry,Wei Yuan,Adam Sharp,Johann de Bono,Andrea Alimonti
View all publications
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Abcam product promise

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