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Anti-IL-23 抗体 [B-Z23] - BSA and Azide free (ab84471)

Reviews (1)Q&A (3)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody [B-Z23] (ab84471)

    Key features and details

    • Mouse monoclonal [B-Z23] to IL-23 - BSA and Azide free
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-IL-23 antibody [B-Z23] - BSA and Azide free
      IL-23 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [B-Z23] to IL-23 - BSA and Azide free

    • 由来種

      Mouse

    • 特異性

      Recognizes both recombinant and natural human IL23, p19 sub-unit.

      Inhibits the bioactivity of rhIL-23 on mouse splenocytes C57BI/6 (measurement of mIL-17 or mIL-22 production).

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant full length protein corresponding to Human IL-23.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.30
      Constituent: 100% PBS

      Sterile-filtered through 0.22 µm. Carrier and preservative free

    • キャリア・フリー

      はい
    • Concentration information loading...

    • 精製度

      Ion Exchange Chromatography

    • 特記事項（精製）

      Sterile-filtered through 0.22 µm and treated to remove endotoxins.

    • ポリ/モノ

      モノクローナル

    • クローン名

      B-Z23

    • ミエローマ

      x63-Ag8.653

    • アイソタイプ

      IgG1

    • 軽鎖の種類

      kappa

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab84471の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    1/500.
    特記事項
    IHC-P
    1/500.

    ターゲット情報

    • 機能

      Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

    • 組織特異性

      Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).

    • 配列類似性

      Belongs to the IL-6 superfamily.

    • 発生段階

      Expressed by newborns dendritic cells.

    • 細胞内局在

      Secreted. Secreted upon association with IL12B.
    • Information by UniProt

    • 参照データベース

      • 別名

        • IL 23 A antibody
        • IL 23 antibody
        • IL 23 subunit alpha antibody
        • IL 23A antibody
        • IL 23p19 antibody
        • IL-23 subunit alpha antibody
        • IL-23-A antibody
        • IL-23p19 antibody
        • IL12B antibody
        • IL23 antibody
        • Il23a antibody
        • IL23A_HUMAN antibody
        • IL23P19 antibody
        • interleukin 12B antibody
        • Interleukin 23 alpha subunit p19 antibody
        • Interleukin 23 p19 subunit antibody
        • interleukin 23 subunit alpha antibody
        • interleukin 23 subunit p19 antibody
        • interleukin six, G CSF related factor antibody
        • Interleukin-23 subunit alpha antibody
        • Interleukin-23 subunit p19 antibody
        • JKA3 induced upon T cell activation antibody
        • MGC79388 antibody
        • P19 antibody
        • SGRF antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody [B-Z23] (ab84471)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody [B-Z23] (ab84471)

        Paraffin embedded human psoriasis lesion tissue labelling IL-23 with ab84471 at 1/500 dilution (Red). DAPI (Blue).

      参考文献 (2)

      ab84471 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab84471 は 2 報の論文で使用されています。

      • Ziblat A  et al. Interleukin (IL)-23 Stimulates IFN-γ Secretion by CD56bright Natural Killer Cells and Enhances IL-18-Driven Dendritic Cells Activation. Front Immunol 8:1959 (2017). PubMed: 29403472
      • Zhang L  et al. IL-23 selectively promotes the metastasis of colorectal carcinoma cells with impaired Socs3 expression via the STAT5 pathway. Carcinogenesis 35:1330-40 (2014). PubMed: 24464786

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-IL23 antibody [B-Z23] - BSA and Azide free

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Monocytes)
      Specification
      Monocytes
      Fixative
      Methanol
      Permeabilization
      Yes - 0,1% Saponin/PBS
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Feb 15 2012

      Question

      Customer kindly contacted us regarding use of this product in a neutralization assay.

      Read More
      Answer

      The lab has provide me with the neutralization protocol used by them. The peptides and reagents are all listed on this. The test is done on murine cells for human cytokine, the mIL-23 and hIL-23 are closed and cross-reacted on cells from mice or human. I have attached this protocol for your review.

      Read More

      Question

      This sounds like a very interesting offer! However, we would prefer to test a monoclonal Antibody. Do you have a monoclonal antibody where the epitope does match the bovine IL-23 sequence?

      With best regards

      Read More
      Answer

      Thank you for contacting us.

      The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more specific; I can however still provide you testing discount code.

      There are 2 mouse monoclonal available in catalogue ab136544 and ab84471. Please choose one of these for Abtrial and let me know.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      What is the protocol for neutralization with this clone?

      Read More
      Answer

      Thank you for contacting us. I have attached the protocol that we received from the laboratory that tested this IL23 antibody for neutralization. The assay measures the amount of IL17 that is produced by mouse splenocytes in response to IL23 and IL2 protein, +/- the anti-IL23 antibody. (Mouse primary splenocytes were used because results with continuous human cell lines were inconclusive). The measurement of IL17 requires an ELISA. The kit from R&D is suggested but we also have a similar kit, a sandwich ELISA for mouse IL17, with the catalogue number ab100702. For IL23 protein, we have ab106889, and for IL2, ab9856. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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