Anti-IL-23 抗体 (ab45420)
Key features and details
- Rabbit polyclonal to IL-23
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-23 antibody
IL-23 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-23 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IL-23 (C terminal). 14 amino acid peptide located within the last 50 amino acids of IL23.
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ポジティブ･コントロール
- Raji cells and Raji cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 10 µg/ml.
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
ターゲット情報
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機能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
組織特異性
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
配列類似性
Belongs to the IL-6 superfamily. -
発生段階
Expressed by newborns dendritic cells. -
細胞内局在
Secreted. Secreted upon association with IL12B. - Information by UniProt
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参照データベース
- Entrez Gene: 3593 Human
- Entrez Gene: 51561 Human
- Omim: 161561 Human
- Omim: 605580 Human
- SwissProt: P29460 Human
- SwissProt: Q9NPF7 Human
- Unigene: 382212 Human
- Unigene: 98309 Human
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別名
- IL 23 A antibody
- IL 23 antibody
- IL 23 subunit alpha antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-23 antibody (ab45420)
Immunohistochemistry of IL-23 in human pancreas tissue with ab45420 at 5 µg/mL.
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Western blot - Anti-IL-23 antibody (ab45420)All lanes : Anti-IL-23 antibody (ab45420) at 1 µg/ml
Lane 1 : Raji lysate
Lane 2 : Raji lysate with IL23 P19 blocking peptide
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-IL23 antibody (ab45420)This image is courtesy of an anonymous AbreviewAb45420 staining IL23 in Mouse bone marrow derived macrophages by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody at 1/200 dilution in 5% goat serum in PBS for 1 hour at 20°C. An Alexa Fluor® 488 goat anti-rabbit was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)ab45420 at 10 µg/ml staining IL23 in Raji cells by ICC/IF -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)This image is courtesy of an Abreview submitted by Dr James Harrisab45420 staining IL23 P19 (green) in Human THP-1 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in 5% goat serum + 1 BSA) for 1 hour at 20°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-IL-23 antibody (ab45420)Immunofluorescence of IL-23 in Raji cells using ab45420 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab45420 は 35 報の論文で使用されています。
- Jin Q et al. MYC promotes fibroblast osteogenesis by regulating ALP and BMP2 to participate in ectopic ossification of ankylosing spondylitis. Arthritis Res Ther 25:28 (2023). PubMed: 36803548
- Nattkemper LA et al. Neuroimmune Mediators of Pruritus in Hispanic Scalp Psoriatic Itch. Acta Derm Venereol 103:adv4463 (2023). PubMed: 36967545
- Di Vincenzo A et al. Insulin Stimulates IL-23 Expression in Human Adipocytes: A Possible Explanation for the Higher Prevalence of Psoriasis in Obesity. Diabetes Metab Syndr Obes 16:1885-1893 (2023). PubMed: 37384131
- Xi L et al. Enhanced uptake and anti-maturation effect of celastrol-loaded mannosylated liposomes on dendritic cells for psoriasis treatment. Acta Pharm Sin B 12:339-352 (2022). PubMed: 35127390
- Ikeda K et al. Obesity and Dyslipidemia Synergistically Exacerbate Psoriatic Skin Inflammation. Int J Mol Sci 23:N/A (2022). PubMed: 35457132