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リコンビナントRabMAb

Anti-IL-21 抗体 [EPR22618-28] (BSA and Azide free) (ab282737)

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Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
  • Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
  • Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22618-28] to IL-21 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free)
    IL-21 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22618-28] to IL-21 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra)more details
    適用なし: IHC-P or WB

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Flow Cyt (Intra): Th17 polarized Mouse splenocytes treated with ionomycin then Brefeldin A; 293T (Human embryonic kidney epithelial cell) transfected with HA tagged IL-21 construct.

  • 特記事項

    ab282737 is the carrier-free version of ab227837.

     

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab282737の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/500.
特記事項
Flow Cyt (Intra)
1/500.
追加情報
Is unsuitable for IHC-P or WB.

ターゲット情報

  • 機能

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.

  • 組織特異性

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.

  • 配列類似性

    Belongs to the IL-15/IL-21 family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CVID11 antibody
      • IL 21 antibody
      • IL-21 antibody
      • Il21 antibody
      • IL21_HUMAN antibody
      • Interleukin 21 antibody
      • Interleukin-21 antibody
      • interleukin-21 isoform antibody
      • Interleukin21 antibody
      • OTTHUMP00000164088 antibody
      • Za11 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
      Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)

      This data was developed using ab227837, the same antibody clone in a different buffer formulation.

      Flow Cytometry (Intracellular) analysis of Th17 polarized mouse splenocytes treated with 500 ng/ml PMA and 500 ng/ml ionomycin for 1 hour, followed by addition of 1 μg/ml Brefeldin A for another 4 hours. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol, and labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).

      IL21 is mutual exclusively expressed with IFN gamma (Th1 marker).

      Cells were provided by an anonymous collaborator.

    • Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
      Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)

      This data was developed using ab227837, the same antibody clone in a different buffer formulation.

      Flow Cytometry (Intracellular) analysis of 293T (human embryonic kidney epithelial cell) transfected with HA-tagged IL-21 construct labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).

    • Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
      Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab282737 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab282737 は論文での使用が確認できていません。

    レビューと Q&A

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