Anti-IL-21 抗体 [EPR22618-28] (ab227837)
![Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (ab227837)](https://www.abcam.co.jp/ps/products/227/ab227837/Images/ab227837-488270-anti-IL21antibody-EPR2261828-flow-cytometry-th17-mouse.jpg "Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (ab227837)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22618-28] to IL-21
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-IL-21 antibody [EPR22618-28]
IL-21 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22618-28] to IL-21 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra)more details
適用なし: IHC-P or WB -
種交差性
交差種: Mouse
非交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt (Intra): Th17 polarized Mouse splenocytes treated with ionomycin then Brefeldin A; 293T (Human embryonic kidney epithelial cell) transfected with HA tagged IL-21 construct.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22618-28 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227837の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
組織特異性
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
配列類似性
Belongs to the IL-15/IL-21 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 60505 Mouse
- SwissProt: Q9ES17 Mouse
- Unigene: 157689 Mouse
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別名
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (ab227837)
Flow Cytometry (Intracellular) analysis of Th17 polarized mouse splenocytes treated with 500 ng/ml PMA and 500 ng/ml ionomycin for 1 hour, followed by addition of 1 μg/ml Brefeldin A for another 4 hours. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol, and labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).
IL21 is mutual exclusively expressed with IFN gamma (Th1 marker).
Cells were provided by an anonymous collaborator.
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![Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (ab227837)](https://www.abcam.co.jp/ps/products/227/ab227837/Images/ab227837-488269-anti-IL21antibody-EPR2261828-flow-cytometry-human-293t.jpg)
Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (ab227837)Flow Cytometry (Intracellular) analysis of 293T (human embryonic kidney epithelial cell) transfected with HA-tagged IL-21 construct labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).
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![Anti-IL-21 antibody [EPR22618-28] (ab227837)](https://www.abcam.co.jp/ps/products/227/ab227837/Images/ab227837-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-21 antibody [EPR22618-28] (ab227837)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab227837 は論文での使用が確認できていません。