Anti-IL-21 抗体 (ab154767)
Key features and details
- Rabbit polyclonal to IL-21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IL-21 antibody
IL-21 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-21 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey
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免疫原
Recombinant fragment corresponding to Human IL-21 aa 1-155.
Database link: Q9HBE4 -
ポジティブ･コントロール
- HL60 whole cell lysate; Human colon carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154767の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
組織特異性
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
配列類似性
Belongs to the IL-15/IL-21 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 59067 Human
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
- Unigene: 567559 Human
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別名
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (ab154767)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling IL21 with ab154767 at 1/500 dilution.
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Western blot - Anti-IL-21 antibody (ab154767)Anti-IL-21 antibody (ab154767) at 1/1000 dilution + HL60 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154767 は 2 報の論文で使用されています。
- Toskas A et al. Expression of IL-21 and IL-33 in Intestinal Mucosa of Inflammatory Bowel Disease: An Immunohistochemical Study. Diagnostics (Basel) 13:N/A (2023). PubMed: 37443579
- Hu J et al. [Immunoexpression and clinical significance of interleukin-21 and receptor activator of nuclear factor ?B ligand in human periapical granulomas and radicular cysts]. Hua Xi Kou Qiang Yi Xue Za Zhi 33:244-8 (2015). PubMed: 26281250