AB150306

Anti-IL-2 Receptor alpha 抗体 [SP176], prediluted

Anti-IL-2 Receptor alpha antibody [SP176], prediluted

RabMAb
Recombinant
詳細を見る

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(1 Publication)

Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human tonsil tissue labelling IL2 Receptor alpha with ab150306.

Unknown

Western blot - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

All lanes:

Western blot - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (ab150306)

All lanes:

MOLT4 cell lysate

Predicted band size: 31 kDa

false

Lab

Western blot - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231441)

Blocking and diluting buffer and concentration : 5% NFDM /TBST

ab181602 was used as a GAPDH loading control.

All lanes:

Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (<a href='/products/primary-antibodies/il-2-receptor-alpha-antibody-sp176-ab231441'>ab231441</a>) at 1/1000 dilution

Lane 1:

HEK-293T transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with a human IL2RA fragment (aa22-272) expression vector containing a His tag whole cell lysate at 20 µg

Lane 3:

HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa,50 kDa

false

Exposure time: 5s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP176

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1", "IHCP-species-notes": "<p>Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

精製に関する特記事項

Purified from TCS by protein A/G.

バッファー組成

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: Tris buffered saline, 1% BSA

出荷温度

Blue Ice

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information IL2RA

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Drug development research 80:824-830 PubMed31301186

2019

Dimethylaminoparthenolide reduces the incidence of dysplasia and ameliorates a wasting syndrome in HPV16-transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Joana M O Santos,Alexandra Moreira-Pais,Tiago Neto,Sara Peixoto da Silva,Paula A Oliveira,Rita Ferreira,Joaquim Mendes,Margarida M S M Bastos,Carlos Lopes,Fátima Casaca,Sandra Silva,Christopher Sweeney,Rui Medeiros,Rui M Gil da Costa

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha 抗体 [SP176], prediluted

Anti-IL-2 Receptor alpha antibody [SP176], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

Western blot - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

Western blot - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (1)

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Abcam product promise