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AB8235

Anti-IL-2 Receptor alpha 抗体 [MEM-181]

Anti-IL-2 Receptor alpha antibody [MEM-181]

5

(1 Review)

|

(7 Publications)

Mouse Monoclonal IL2RA antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing IL2RA protein.

別名を表示する

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

2 Images
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Overlay histogram showing Jurkat cells stained with ab8235 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8235, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed Jurkat cells used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-2 Receptor alpha antibody [MEM-181] (AB8235)

Flow cytometry analysis showing separation of human PHA stimulated peripheral blood mononuclear cells stained using ab8235 (concentration in sample 2 µg/ml, GAM APC, red-filled) from human PHA stimulated peripheral blood mononuclear cells unstained by primary antibody (GAM APC, black-dashed).

関連する標識済み抗体及び組成の異なる製品 (7)

  • 660 APC

    APC Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 785 APC/Cy7®

    APC/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 519 FITC

    FITC Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 578 PE

    PE Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 778 PE/Cy7®

    PE/Cy7® Anti-IL-2 Receptor alpha antibody [MEM-181]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-IL-2 Receptor alpha antibody [MEM-181]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-181

アイソタイプ

IgG1

軽鎖のタイプ

unknown

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing IL2RA protein. The exact immunogen used to generate this antibody is proprietary information.

P01589

エピトープ

extracellular

特異性

CD25 antigen (human).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified from TCS. Purity >95% by SDS-PAGE.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
See full target information IL2RA
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:1951-1964 PubMed39963418

2025

Functionalized Carbon Dots With Intrinsic Wnt/-Catenin Inhibition to Synergistically Promote 5-Fluorouracil Chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ziwei Yang,Mingyue Zhou,Tianpeng Yin,Cai-Yun Wang,Guo-Yuan Zhu,Li-Ping Bai,Zhi-Hong Jiang,Wei Zhang

Molecular brain 15:18 PubMed35183222

2022

The adaptor protein PICK1 targets the sorting receptor SorLA.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Binkle,Marcel Klein,Uwe Borgmeyer,Dietmar Kuhl,Guido Hermey

Environmental toxicology and pharmacology 80:103434 PubMed32544427

2020

Combined effects of BPA and PFOS on fetal cardiac development: In vitro and in vivo experiments.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Zhou,Wei Cheng,Yan Feng,Wei Wang,Fan Liang,Fei Luo,Shoufei Yang,Yan Wang

Cancer science 110:2507-2519 PubMed31215741

2019

Interaction of transforming growth factor-β-Smads/microRNA-362-3p/CD82 mediated by M2 macrophages promotes the process of epithelial-mesenchymal transition in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Zhang,Feng Huang,Yongliang Yao,Jianjun Wang,Jue Wei,Qiong Wu,Shihao Xiang,Ling Xu

The Journal of comparative neurology 522:3386-402 PubMed24715575

2014

Spatiotemporal expression analysis of the growth factor receptor SorCS3.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Oetjen,Claudia Mahlke,Irm Hermans-Borgmeyer,Guido Hermey

PloS one 8:e62435 PubMed23626818

2013

Cell surface localization of α3β4 nicotinic acetylcholine receptors is regulated by N-cadherin homotypic binding and actomyosin contractility.

Applications

ICC/IF

Species

Human

Juan L Brusés

Traffic (Copenhagen, Denmark) 9:980-94 PubMed18315530

2008

Different motifs regulate trafficking of SorCS1 isoforms.

Applications

ICC/IF

Species

Mouse

Morten S Nielsen,Sady J Keat,Jida W Hamati,Peder Madsen,Jakob J Gutzmann,Arne Engelsberg,Karen M Pedersen,Camilla Gustafsen,Anders Nykjaer,Jørgen Gliemann,Irm Hermans-Borgmeyer,Dietmar Kuhl,Claus M Petersen,Guido Hermey
View all publications
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代替製品

Primary Antibodies

AB231441

Anti-IL-2 Receptor alpha antibody [SP176]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (AB231441)

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Primary Antibodies

AB207895

Alexa Fluor® 594 Anti-IL-2 Receptor alpha antibody [EPR6452]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-IL-2 Receptor alpha antibody [EPR6452] (AB207895)

  • 0
  • 0
Primary Antibodies

AB128955

Anti-IL-2 Receptor alpha antibody [EPR6452]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (AB128955)

  • 0
  • 0
Primary Antibodies

AB238272

Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] - BSA and Azide free (AB238272)

  • 0
  • 0
Primary Antibodies

AB150306

Anti-IL-2 Receptor alpha antibody [SP176], prediluted

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176], prediluted (AB150306)

  • 0
  • 0
Primary Antibodies

AB215378

Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free (AB215378)

  • 1
  • 1

組合せ製品

Assay Kits

AB283543

Human IL-2 Receptor alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-2 Receptor alpha ELISA Kit (AB283543)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB20034

Anti-FOXP3 antibody [236A/E7]

BOND RX™ Validated|Recombinant|Lab Essentials|Advanced Validation

Multiplex immunohistochemistry - Anti-FOXP3 antibody [236A/E7] (AB20034)

  • 4
  • 38

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