AB227834

Anti-IL-2 Receptor alpha 抗体 [EPR22588-18]

Name: Anti-IL-2 Receptor alpha 抗体 [EPR22588-18] (ab227834) | Abcam
Brand: Abcam Limited
SKU: AB227834
Price: 30000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-IL-2 Receptor alpha antibody [EPR22588-18]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
詳細を見る

(2 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal IL2RA antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

別名を表示する

CD25, Il2r, Il2ra, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, p55

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of rat spleen (PMID : 17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL-2 Receptor alpha with ab227834 at 1/500 dilution (1.26 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on T cells of mouse spleen (PMID : 17056509) is observed. The section was incubated with ab227834 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

IHC-P

AbReview79362****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of formaldehyde-fixed mouse CT26 tumor taken out of Balb/C mice tissue. Stained with ab227834 at 1/500 dilution. Secondary antibody used was ImmPRESS® HRP Goat Anti-Rabbit IgG Polymer Detection. Blocking was done with Peroxidase Blocking Solution, BLOXALL for 20 minutes at 20°C. The sample was incubated with the primary antibody and Signalstain ab diluent for 18 hours at 4°C. Antigen retrieval method was heat mediated with Tris-EDTA pH 9.

This image is courtesy of an anonymous Abreview

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR22588-18

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat

アプリケーション

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information Il2ra

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Chemical biology & drug design 106:e70169 PubMed40944385

2025

Kisspeptin-10 Improves Gestational Diabetes Mellitus Symptoms in Rats by Suppressing Insulin Resistance in Placental Trophoblast Cells by Activating the Cyclic AMP/Protein Kinase A Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhua Li,Jinhuan Chen,Lin Lu,Bei Gan

Cell reports 43:114819 PubMed39368087

2024

PD-L1 restrains PD-1Nrp1 Treg cells to suppress inflammation-driven colorectal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dakota B Poschel,John D Klement,Alyssa D Merting,Chunwan Lu,Yang Zhao,Dafeng Yang,Wei Xiao,Huabin Zhu,Ponnala Rajeshwari,Michael Toscano,Kimya Jones,Amanda Barrett,Roni J Bollag,Padraic G Fallon,Huidong Shi,Kebin Liu

Cell genomics 4:100659 PubMed39317187

2024

CXCR4 orchestrates the TOX-programmed exhausted phenotype of CD8 T cells via JAK2/STAT3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Canhui Cao,Miaochun Xu,Ye Wei,Ting Peng,Shitong Lin,Xiaojie Liu,Yashi Xu,Tian Chu,Shiyi Liu,Ping Wu,Bai Hu,Wencheng Ding,Li Li,Ding Ma,Peng Wu

Cell communication and signaling : CCS 22:429 PubMed39227807

2024

NDR1 mediates PD-L1 deubiquitination to promote prostate cancer immune escape via USP10.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Fu,Jinxin Li,Zuodong Xuan,Zeyuan Zheng,Yankuo Liu,Zeyi Zhang,Jianzhong Zheng,Min Zhong,Bin Liu,Yifan Du,Lei Zhang,Huimin Sun

Human gene therapy 33:649-663 PubMed35272497

2022

Bispecific Antibody Expressed by an Oncolytic Herpes Simplex Virus Type 2 Can Transform Heterologous T Cells Into Uniform Tumor Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Jin,Runyang Wang,Junhan Yang,Han Hu,Di Wang,Linkang Cai,Zhizheng Fang,Shuang Dong,Sheng Hu,Yang Wang,Binlei Liu

Cancer communications (London, England) 42:17-36 PubMed34981670

2022

Anti-PD-L1/TGF-βR fusion protein (SHR-1701) overcomes disrupted lymphocyte recovery-induced resistance to PD-1/PD-L1 inhibitors in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Cheng,Kaikai Ding,Pengxiang Chen,Jianxiong Ji,Tao Luo,Xiaofan Guo,Wei Qiu,Chunhong Ma,Xue Meng,Jian Wang,Jinming Yu,Yuan Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-2 Receptor alpha 抗体 [EPR22588-18]

Anti-IL-2 Receptor alpha antibody [EPR22588-18]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR22588-18] (AB227834)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise