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リコンビナントRabMAb

Anti-IL-17A 抗体 [EPR26410-51] (BSA and Azide free) (ab302923)

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Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
  • Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
  • Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
  • Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26410-51] to IL-17A - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

製品の概要

  • 製品名

    Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free)
    IL-17A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26410-51] to IL-17A - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, Flow Cyt (Intra)more details
    適用なし: IHC-P,IP or WB

  • 種交差性

    交差種: Mouse
    非交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: EL4 (mouse lymphoma T lymphocytes) Flow Cyt (Intra): EL4 (mouse lymphoma T lymphocytes); mouse thymocytes treated with PMA and Ionomycin Ca2+ Salt and Brefeldin.

  • 特記事項

    ab302923 is the carrier-free version of ab302922.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26410-51

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302923の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P,IP or WB.

ターゲット情報

  • 機能

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.

  • 組織特異性

    Restricted to activated memory T-cells.

  • 配列類似性

    Belongs to the IL-17 family.

  • 翻訳後修飾

    Found both in glycosylated and nonglycosylated forms.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CTLA 8 antibody
      • CTLA-8 antibody
      • CTLA8 antibody
      • cytotoxic T lymphocyte associated antigen 8 antibody
      • Cytotoxic T lymphocyte associated protein 8 antibody
      • Cytotoxic T lymphocyte associated serine esterase 8 antibody
      • Cytotoxic T-lymphocyte-associated antigen 8 antibody
      • IL 17 antibody
      • IL 17A antibody
      • IL-17 antibody
      • IL-17A antibody
      • IL17 antibody
      • IL17_HUMAN antibody
      • Il17a antibody
      • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
      • interleukin 17A antibody
      • Interleukin-17A antibody
      • interleukin17 antibody
      • Interleukin17A antibody
      • OTTHUMP00000016597 antibody
      • OTTMUSP00000046003 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)

      This data was developed using ab302922, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labeling IL-17A with ab302922 at 1/500 (0.93 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing increased cytoplasmic staining in EL4 cells treated with Phorbol-12-myristate-13-acetate (100 ng/mL) and Brefeldin A (500 ng/mL) overnight is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

    • Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
      Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)

      This data was developed using ab302922, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized EL4 (mouse lymphoma T lymphocyte) treated with 50ng/ml PMA and 500ng/ml Ionomycin Ca2+ Salt and 500ng/ml Brefeldin A overnight (Right) / Untreated control (Left). Primary antibody concentration 1/500 dilution (0.1μg). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

    • Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
      Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)

      This data was developed using ab302922, the same antibody clone in a different buffer formulation.

      Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized mouse thymocytes treated with 50ng/ml PMA and 500ng/ml Ionomycin Ca2+ Salt and 500ng/ml Brefeldin A overnight (Right) / Untreated control (Left). ab302922 at 1/500 dilution (0.1ug). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with ab302922.

    • Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)
      Anti-IL-17A antibody [EPR26410-51] (BSA and Azide free) (AB302923)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302923 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302923 は論文での使用が確認できていません。

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