Anti-IL-17A 抗体 (ab79056)
Key features and details
- Rabbit polyclonal to IL-17A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-17A antibody
IL-17A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-17A -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
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ポジティブ･コントロール
- IHC-P: lymphoid nodules
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特記事項
The blocking peptide is available as ab190773
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 5 - 20 µg/ml.
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| IHC-P | (9) |
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
Use a concentration of 0.125 - 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Can be blocked with IL-17A peptide (ab190773).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
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IHC-P
Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.125 - 0.5 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).Can be blocked with IL-17A peptide (ab190773). |
ターゲット情報
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機能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
組織特異性
Restricted to activated memory T-cells. -
配列類似性
Belongs to the IL-17 family. -
翻訳後修飾
Found both in glycosylated and nonglycosylated forms. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3605 Human
- Entrez Gene: 16171 Mouse
- Omim: 603149 Human
- SwissProt: Q16552 Human
- SwissProt: Q62386 Mouse
- Unigene: 41724 Human
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別名
- CTLA 8 antibody
- CTLA-8 antibody
- CTLA8 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)
Immunohistochemical analysis of paraffin-embedded Mouse Colon Tissue using ab79056 at 2 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-17A antibody (ab79056)Immunocytochemistry/Immunofluorescence analysis of A-20 (mouse reticulum sarcoma cell line) cells labelling IL-17A with purified ab79056 at 5 µg/mL. Cells were fixed with 4% paraformaldehyde. A goat anti-rabbit IgG (1/500) was used as the secondary antibody (green). Nuclei counterstained with DAPI (blue).
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Western blot - Anti-IL-17A antibody (ab79056)Lane 1 : Anti-IL-17A antibody (ab79056) at 0.5 µg/ml
Lane 2 : Anti-IL-17A antibody (ab79056) at 1 µg/ml
All lanes : A-20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Additional bands at: 22 kDa, 28 kDa, 32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL-17A.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using ab79056 at 5 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)This image is courtesy of an Abreview submitted by Claudia Zizzadoroab79056 staining IL-17A in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-17A antibody (ab79056)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thymus tissue staining IL-17A with ab79056 at 20µg/ml. Goat anti-rabbit IgG (1/500) was used as the secondary antibody (red).
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Western blot - Anti-IL-17A antibody (ab79056)All lanes : Anti-IL-17A antibody (ab79056)
Lane 1 : human thymus tissue lysate at 1 µg/ml
Lane 2 : human thymus tissue lysate at 2 µg/ml
Predicted band size: 18 kDa -
Western blot - Anti-IL-17A antibody (ab79056)Anti-IL-17A antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg
Predicted band size: 18 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (183)
ab79056 は 183 報の論文で使用されています。
- Liang X et al. Xuanhuang Runtong Tablets Relieve Slow Transit Constipation in Mice by Regulating TLR5/IL-17A Signaling Mediated by Gut Microbes. Evid Based Complement Alternat Med 2023:6506244 (2023). PubMed: 36700038
- Cui B et al. Dendritic cells originating exosomal miR-193b-3p induces regulatory T cells to alleviate liver transplant rejection. Int Immunopharmacol 114:109541 (2023). PubMed: 36700764
- Zhang Q et al. Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis. Front Immunol 14:1107559 (2023). PubMed: 36742316
- Lee AR et al. SARS-CoV-2 spike protein promotes inflammatory cytokine activation and aggravates rheumatoid arthritis. Cell Commun Signal 21:44 (2023). PubMed: 36864432
- Hayakawa K et al. Topical application of imatinib mesylate ameliorated psoriasis-like skin lesions in imiquimod-induced murine model via angiogenesis inhibition. Exp Dermatol 32:878-888 (2023). PubMed: 36928929