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AB324048

Anti-IL-13 receptor alpha 2 抗体 [EPR29921-581]

Anti-IL-13 receptor alpha 2 antibody [EPR29921-581]

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Rabbit Recombinant Monoclonal IL-13 receptor alpha 2 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

別名を表示する

CD213a2, IL13R, IL13RA2, Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13RA2, Interleukin-13-binding protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Wild-type A375 cells and IL13RA2 KO A375 cells labelling IL-13 receptor alpha 2 with ab324048 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in parental A375 cell line (shown in green).

The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-alpha Tubulin mouse monoclonal antibody (ab7291) was used to counterstain tubulin at 1/1000 dilution (Magenta).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution.

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of A-172 (human brain glioblastoma cell, Right) / Daudi (human Burkitt's lymphoma lymphoblast, Left) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Right compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : Daudi.
Gated on viable cells.

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-IL-13 receptor alpha 2 antibody [EPR29921-581] (AB324048)

Flow cytometric analysis of IL13RA2 KO A375 (IL-13 receptor alpha 2 knock out human malignant melanoma epithelial cell, Magenta) / A375 (Green) cells labelling IL-13 receptor alpha 2 with ab324048 at 1/500 dilution (0.1 ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR29921-581

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cell surface receptor that plays a role in the regulation of IL-13-mediated responses (PubMed : 11861389, PubMed : 17030238). Functions as a decoy receptor that inhibits IL-13- and IL-4-mediated signal transduction via the JAK-STAT pathway and thereby modulates immune responses and inflammation (PubMed : 11861389, PubMed : 17030238). Serves as a functional signaling receptor for IL-13 in an alternative pathway involving AP-1 ultimately leading to the production of TGFB1 (PubMed : 16327802).
See full target information IL13RA2
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組合せ製品

Assay Kits

AB274400

Human IL-13R alpha 2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (AB274400)

  • 0
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Abcam product promise

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