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AB285053

Anti-IL-13 抗体 [EPR20465-131] - BSA and Azide free (Capture)

Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture)

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Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture) (ab285053) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting IL-13 in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

NC30, IL13, Interleukin-13, IL-13

1 Images
Sandwich ELISA - Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture) (AB285053)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-IL-13 antibody [EPR20465-131] - BSA and Azide free (Capture) (AB285053)

Example of Human IL-13 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR20465-131

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human, Monkey

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

ab285053 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL-13. The recommended pair for sandwich ELISA is:
Capture: ab285053, Human IL-13 Capture Antibody (unconjugated)
Detector: ab285159, Human IL-13 Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed : 8096327, PubMed : 8097324). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed : 8096327). Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (PubMed : 7903680, PubMed : 8759755). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Also functions on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils (PubMed : 8639787). Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed : 9013879). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed : 21622864).
See full target information IL13
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