Anti-IL-12RB2 抗体 [EPR24604-33] - BSA and Azide free (Detector) (ab289811)
![Sandwich ELISA - Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector) (ab289811)](https://www.abcam.co.jp/ps/products/289/ab289811/Images/ab289811-2-human-il-12rb2-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector) (ab289811)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24604-33] to IL-12RB2 - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Human
製品の概要
-
製品名
Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector)
IL-12RB2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24604-33] to IL-12RB2 - BSA and Azide free (Detector) -
由来種
Rabbit -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab289811 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL-12RB2. The recommended pair for sandwich ELISA is:
Capture: ab289810, Human IL-12RB2 Capture Antibody (unconjugated)
Detector: ab289811, Human IL-12RB2 Detector Antibody (unconjugated)
The reference range value is 0.3125-10 ng/mL.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24604-33 -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24604-120] to IL-12RB2 - BSA and Azide free (Capture) (ab289810).
|
| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24604-120] to IL-12RB2 - BSA and Azide free (Capture) (ab289810). |
ターゲット情報
-
機能
Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. -
組織特異性
Isoform 2 is expressed at similar levels in both naive and activated T-cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains. -
発生段階
Maximum levels in Th1 cells between day 3 and day 8 of activation. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
On IL12 binding, phosphorylated on C-terminal tyrosine residues by JAK2. Phosphorylation on Tyr-800 is required for STAT4 binding and activation, and for SOCS3 binding. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 3595 Human
- Omim: 601642 Human
- SwissProt: Q99665 Human
- Unigene: 479347 Human
-
別名
- I12R2_HUMAN antibody
- IL-12 receptor subunit beta-2 antibody
- IL-12R subunit beta-2 antibody
see all
画像
-
Sandwich ELISA - Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector) (ab289811)Sandwich ELISA of ab289800 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
-
![Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector) (ab289811)](https://www.abcam.co.jp/ps/products/289/ab289811/Images/ab289811-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-12RB2 antibody [EPR24604-33] - BSA and Azide free (Detector) (ab289811)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab289811 は論文での使用が確認できていません。
