Anti-IL-12 抗体 [AP-MAB0853] (ab80682)
Key features and details
- Rat monoclonal [AP-MAB0853] to IL-12
- Suitable for: IP, Neutralising, Flow Cyt, WB
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-IL-12 antibody [AP-MAB0853]
IL-12 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [AP-MAB0853] to IL-12 -
由来種
Rat -
アプリケーション
適用あり: IP, Neutralising, Flow Cyt, WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment corresponding to Mouse IL-12.
Database link: P43431 -
特記事項
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).ab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
一次抗体 備考
ab80682 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat). -
ポリ/モノ
モノクローナル -
クローン名
AP-MAB0853 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80682の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| Neutralising |
Use at an assay dependent concentration.
Neutralizes the bioactivity of natural or recombinant IL12 both in vivo and in vitro. |
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IP
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. Neutralizes the bioactivity of natural or recombinant IL12 both in vivo and in vitro. |
|
Flow Cyt
Use at an assay dependent concentration. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
配列類似性
Belongs to the IL-6 superfamily. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16159 Mouse
- Entrez Gene: 16160 Mouse
- SwissProt: P43431 Mouse
- SwissProt: P43432 Mouse
- Unigene: 103783 Mouse
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別名
- CLMF antibody
- CLMF p35 antibody
- CLMF p40 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab80682 は 3 報の論文で使用されています。
- Crespo M et al. Myeloid p38 activation maintains macrophage-liver crosstalk and BAT thermogenesis through IL-12-FGF21 axis. Hepatology 77:874-887 (2023). PubMed: 35592906
- Yamaguchi T et al. Proinflammatory M1 Macrophages Inhibit RANKL-Induced Osteoclastogenesis. Infect Immun 84:2802-12 (2016). PubMed: 27456834
- Zhou X et al. Analysis of the direct injury effector of oligodendroglia cells or myelin sheath in an experimental allergic encephalomyelitis model induced by the MOG35-55 peptide. Mol Med Rep 12:7425-32 (2015). PubMed: 26459920
