Anti-IL-10 抗体 (ab34843)
Key features and details
- Rabbit polyclonal to IL-10
- Suitable for: RIA, Neutralising, WB, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-IL-10 antibody
IL-10 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-10 -
由来種
Rabbit -
特異性
The antiserum detects recombinant and native IL-10 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-10 production from fibroblasts). The antibody recognizes hu IL-10. The antibody will not neutralize the biological activity of murine IL-10.
-
アプリケーション
適用あり: RIA, Neutralising, WB, ELISAmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Full length native protein (purified) corresponding to Human IL-10.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
|
|
| Neutralising |
Use at an assay dependent concentration.
|
|
| WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
| ELISA |
Use at an assay dependent concentration.
(as detection antibody, using a monoclonal antibody for capture). |
| 特記事項 |
|---|
|
RIA
Use at an assay dependent concentration. |
|
Neutralising
Use at an assay dependent concentration. |
|
WB
1/2000 - 1/10000. Predicted molecular weight: 21 kDa. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-10 will appear due to the variable amount of glycosylation on the molecule. |
|
ELISA
Use at an assay dependent concentration. (as detection antibody, using a monoclonal antibody for capture). |
ターゲット情報
-
機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 3586 Human
- Omim: 124092 Human
- SwissProt: P22301 Human
- Unigene: 193717 Human
-
別名
- CSIF antibody
- Cytokine synthesis inhibitory factor antibody
- GVHDS antibody
see all
画像
-
Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/200 dilution + recombinant IL-10 protein
Secondary
Goat anti-rabbit at 1/20000 dilution
Predicted band size: 21 kDa
4-20% reducing gel.Membrane was blocked in 3% BSA-TBS for 1 hour 4°C.Dilution buffer: 3% BSA-TBS.Incubated O/N at 4°C. -
Western blot - Anti-IL-10 antibody (ab34843)Anti-IL-10 antibody (ab34843) at 1/500 dilution + recombinant IL-10 protein, 50ng
Secondary
Peroxidase conjugated secondary at 1/40000 dilution
Predicted band size: 21 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (91)
ab34843 は 91 報の論文で使用されています。
- Sandoval Pacheco CM et al. Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi. Parasite Immunol 45:e12971 (2023). PubMed: 36695719
- Gál L et al. Restoration of Motor Function through Delayed Intraspinal Delivery of Human IL-10-Encoding Nucleoside-Modified mRNA after Spinal Cord Injury. Research (Wash D C) 6:0056 (2023). PubMed: 36930811
- Xie Q et al. Chitosan oligosaccharide improves ovarian granulosa cells inflammation and oxidative stress in patients with polycystic ovary syndrome. Front Immunol 14:1086232 (2023). PubMed: 36936973
- Lvova MN et al. Curcumin and Its Supramolecular Complex with Disodium Glycyrrhizinate as Potential Drugs for the Liver Fluke Infection Caused by Opisthorchis felineus. Pathogens 12:N/A (2023). PubMed: 37375509
- Moyano A et al. EBV Impact in Peripheral Macrophages' Polarization Cytokines in Pediatric Patients. Viruses 15:N/A (2023). PubMed: 37896882