Anti-IL-1 beta 抗体 (ab2105)
Key features and details
- Rabbit polyclonal to IL-1 beta
- Suitable for: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFr
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IL-1 beta antibody
IL-1 beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IL-1 beta -
由来種
Rabbit -
特異性
In general, this antibody also detects primate IL-1 beta in the same formats using similar dilutions. The antiserum does not recognize human IL-1 alpha. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1 beta exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1 beta precursor in lysates.
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アプリケーション
適用あり: RIA, ELISA, ICC/IF, IHC-Fr, Functional Studies, WB, IP, Neutralising, Flow Cyt, IHC-P, IHC-FoFrmore details -
種交差性
交差種: Human
交差が予測される動物種: Non human primates
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免疫原
Recombinant full length protein corresponding to Human IL-1 beta aa 100 to the C-terminus. Produced in E.coli. MW of recombinant IL-1 beta 17kDa, mature chain without propeptide.
Database link: P01584 -
ポジティブ･コントロール
- IHC-P: human medullary lymph node
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特記事項
caution, F(c) domain of rabbit IgG may interact with cells non-specifically.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
DEAE-Chromatography -
特記事項（精製）
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2105の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| RIA |
Use at an assay dependent concentration.
|
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| ELISA | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| Functional Studies |
Use at an assay dependent concentration.
|
|
| WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.
1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
| IP |
Use at an assay dependent concentration.
1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
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| Neutralising |
Use at an assay dependent concentration.
Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
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| Flow Cyt | (1) |
Use at an assay dependent concentration.
F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| IHC-P | (9) |
Use at an assay dependent concentration.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 28726778
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| 特記事項 |
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RIA
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Functional Studies
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 30 kDa. 1/1000 - 1/2000 (antigen - supernatants or lysates of 2 x 106 endotoxin-stimulated human PBMC. Denatured 31kDa precursor IL-1beta will be recognized, but often migrates as a 35 kDa band). |
|
IP
Use at an assay dependent concentration. 1/400 - 1/800 (pre-clearing with a non-specific rabbit IgG is helpful to reduce background). |
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Neutralising
Use at an assay dependent concentration. Neutralization of IL-1beta activity in bioassays 1/100 ( >4 hours incubation, normal rabbit IgG as negative control). ab2105 does not neutralize the biological activity of murine, rat or rabbit IL1 beta. |
|
Flow Cyt
Use at an assay dependent concentration. F(c) domain of rabbit IgG may interact with cells non-specifically! ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 28726778 |
ターゲット情報
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機能
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. -
組織特異性
Expressed in activated monocytes/macrophages (at protein level). -
配列類似性
Belongs to the IL-1 family. -
翻訳後修飾
Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. -
細胞内局在
Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive. - Information by UniProt
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参照データベース
- Entrez Gene: 3553 Human
- Omim: 147720 Human
- SwissProt: P01584 Human
- Unigene: 126256 Human
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別名
- Catabolin antibody
- H1 antibody
- IFN beta inducing factor antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)Immunohistochemical analysis of Formalin-fixed paraffin-embedded human medullary lymph node sections labeling IL-1 beta with ab2105. Peroxidase goat anti-rabbit at 1/10,000 was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 beta antibody (ab2105)This image is courtesy of an Abreview submitted by Miss Silke Vorwaldab2105 at 1/20 staining human liver tissue sections by IHC-P. The cells were paraformaldehyde fixed and a heat mediated antigen retrieval step was performed. The tissue was incubated with the tissue overnight at room temperature. A biotinylated donkey anti-rabbit IgG was used as the secondary antibody and this was detected using streptavidin HRP.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (198)
ab2105 は 198 報の論文で使用されています。
- Huang ZX et al. Lipoxin A4 depresses inflammation and promotes autophagy via AhR/mTOR/AKT pathway to suppress endometriosis. Am J Reprod Immunol 89:e13659 (2023). PubMed: 36412044
- Wang G et al. Commensal microbiome promotes hair follicle regeneration by inducing keratinocyte HIF-1α signaling and glutamine metabolism. Sci Adv 9:eabo7555 (2023). PubMed: 36598999
- Silwal P et al. Chemical mimetics of the N-degron pathway alleviate systemic inflammation by activating mitophagy and immunometabolic remodeling. Exp Mol Med 55:333-346 (2023). PubMed: 36720915
- Owembabazi E et al. Co-administration of alcohol and combination antiretroviral therapy (cART) in male Sprague Dawley rats: A study on testicular morphology, oxidative and cytokines perturbations. Anat Cell Biol 56:236-251 (2023). PubMed: 36759974
- AlQot HE & Rylett RJ A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability. Sci Rep 13:3037 (2023). PubMed: 36810877
