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リコンビナントRabMAb

Anti-IL-1 alpha 抗体 [EPR25263-3] - BSA and Azide free (ab300502)

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Western blot - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
  • Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25263-3] to IL-1 alpha - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free
    IL-1 alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25263-3] to IL-1 alpha - BSA and Azide free

  • 由来種

    Rabbit

  • 特異性

    Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB.

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra) or IP

  • 種交差性

    交差種: Mouse, Human
    非交差種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: RAW264.7 treated with 10µg/ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate IHC-P: Mouse lung treated with lipopolysaccharides (1 µg/ml) for 16 h in vitro, human colon, and human colon cancer. ICC/IF: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 10 ug/ml lipopolysaccharide

  • 特記事項

    ab300502 is a carrier free version of ab300501. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300502の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.

Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB.
IHC-P
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.

Human species reactivity is recommended based on IHC results, we do not guarantee human species reactivity in WB.
IHC-P
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra) or IP.

ターゲット情報

  • 機能

    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

  • 配列類似性

    Belongs to the IL-1 family.

  • ドメイン

    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

  • 細胞内局在

    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt

  • 参照データベース

    • 別名

      • BAF antibody
      • FAF antibody
      • Hematopoietin 1 antibody
      • Hematopoietin-1 antibody
      • IL 1 alpha antibody
      • IL 1A antibody
      • IL-1 alpha antibody
      • Il-1a antibody
      • IL1 ALPHA antibody
      • IL1 antibody
      • IL1A antibody
      • IL1A_HUMAN antibody
      • IL1F1 antibody
      • Interleukin 1 alpha antibody
      • Interleukin-1 alpha antibody
      • Interleukin1 alpha antibody
      • LAF antibody
      • LEM antibody
      • Preinterleukin 1 alpha antibody
      • Pro interleukin 1 alpha antibody
      see all

    画像

    • Western blot - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Western blot - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      All lanes : Anti-IL-1 alpha antibody [EPR25263-3] (ab300501) at 1/1000 dilution

      Lane 1 : Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : RAW264.7 treated with 10µg/ml lipopolysaccharide (LPS) for 7 hours, whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 15 seconds


      This data was developed using ab300501, the same antibody clone in a different buffer formulation. 

      Blocking and dilution buffer and concentration: 5% NFDM/TBST. 

      The expression level of IL-1 alpha was upregulated by LPS stimulation (PMID: 25870118).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

      This data was developed using ab300501, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded A: Mouse lung treated with lipopolysaccharides (1 μg/ml) for 16 h in vitro. B: Untreated mouse lung labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Positive staining on mouse lung induced by LPS (A) and no staining on untreated mouse lung (B). The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

      This data was developed using ab300501, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded human colon tissue labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on immune cells of human colon is observed. The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

      This data was developed using ab300501, the same antibody clone in a different buffer formulation. 

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labelling IL-1 alpha with ab300501 at 1/5000 dilution followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Cytoplasmic staining on immune cells of human colon cancer is observed. The section was incubated with ab300501 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins is used. 

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

      This data was developed using ab300501, the same antibody clone in a different buffer formulation. 

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized treated or untreated for 24h with 10 μg/ml lipopolysaccharide RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate labeling IL-1 alpha with ab300501 at 1/50 dilution, followed by (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining in Raw 264.7 cells treated with lipopolysaccharide (10 μg/ml) for 24 h (PMID:25870118). The nuclear counter stain is DAPI (blue).

      Tubulin is detected with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594, ab195889 ) at 1/200 dilution (red). 

      Secondary antibody only controls: PBS was used instead of primary antibody in lipopolysacchride treated or untreated for 24h RAW 264.7 followed by preadsorbed (ab150081) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution and nuclear stained with DAPI. 

    • Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)
      Anti-IL-1 alpha antibody [EPR25263-3] - BSA and Azide free (ab300502)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300502 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300502 は論文での使用が確認できていません。

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