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AB237037

Anti-IL-1 alpha 抗体 [EPR19904-247] - BSA and Azide free

Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

別名を表示する

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

1 Images
Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (AB237037)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free (AB237037)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green)labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab206410).

関連する標識済み抗体及び組成の異なる製品 (2)

  • Unconjugated

    Anti-IL-1 alpha antibody [EPR19904-247]

  • Carrier free

    Anti-IL-1 alpha antibody [EPR19904-247] - Low endotoxin, Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19904-247

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB9614

Anti-IL-1 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (AB9614)

  • 4
  • 3
Primary Antibodies

AB7632

Anti-IL-1 alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody (AB7632)

  • 3
  • 4
Primary Antibodies

AB239517

Anti-IL-1 alpha antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [C1] (AB239517)

  • 0
  • 0
Primary Antibodies

AB239584

Anti-IL-1 alpha antibody [C5]

Western blot - Anti-IL-1 alpha antibody [C5] (AB239584)

  • 0
  • 0
Proteins & Peptides

AB256050

Recombinant mouse IL-1 alpha protein (Active)

Bioactive

Functional Studies - Recombinant mouse IL-1 alpha protein (Active) (AB256050)

  • 0
  • 0

組合せ製品

Assay Kits

AB178008

Human IL-1a ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-1a ELISA Kit (AB178008)

  • 0
  • 0
Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

  • 0
  • 0
Primary Antibodies

AB215191

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)

  • 5
  • 4
Primary Antibodies

AB215203

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (AB215203)

  • 5
  • 4
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 7
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

ab237037 is the carrier-free version of ab206410.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).
See full target information IL1A
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Neoplasia (New York, N.Y.) 32:100825 PubMed35901621

2022

Longitudinal examination of perfusion and angiogenesis markers in primary colorectal tumors shows distinct signatures for metronomic and maximum-tolerated dose strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel I Mundo,Abdussaboor Muhammad,Kerlin Balza,Christopher E Nelson,Timothy J Muldoon
View all publications
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Abcam product promise

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