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AB206410

Anti-IL-1 alpha 抗体 [EPR19904-247]

Anti-IL-1 alpha antibody [EPR19904-247]

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(5 Publications)

Rabbit Recombinant Monoclonal IL-1 alpha antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

別名を表示する

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

3 Images
Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized THP-1 (human monocytic leukemia cell line) cell line treated with 50 nM phorbol-12-myristate-13-acetate (PMA, ab120297) and 5 μg/ml lipopolysaccharide (LPS) for 24 hours (red) or an untreated control (green)labeling IL-1 alpha with ab206410 at 1/60 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluorr® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • WB

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

Expression of IL-1 alpha can be induced by LPS in macrophages. The molecular masses observed are consistent with the literature (PMID : 9785472; PMID : 1868253; PMID : 19104081).

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human monocytic leukemia cell line) whole cell lysate at 20 µg

Lane 2:

THP-1, treated with 50 nM phorbol-12-myristate-13-acetate (PMA, <a href='/products/biochemicals/phorbol-12-myristate-13-acetate-pma-pkc-activator-ab120297'>ab120297</a>) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 31 kDa

Observed band size: 15 kDa,20 kDa,34 kDa

false

Exposure time: 15s

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)
  • WB

Supplier Data

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (AB206410)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL-1 alpha antibody [EPR19904-247] (ab206410) at 1/1000 dilution

All lanes:

Human IL-1 alpha recombinant protein (aa113-271) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

false

Exposure time: 3s

関連する標識済み抗体及び組成の異なる製品 (2)

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    Anti-IL-1 alpha antibody [EPR19904-247] - BSA and Azide free

  • Carrier free

    Anti-IL-1 alpha antibody [EPR19904-247] - Low endotoxin, Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19904-247

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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代替製品

Primary Antibodies

AB9614

Anti-IL-1 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody (AB9614)

  • 4
  • 3
Primary Antibodies

AB7632

Anti-IL-1 alpha antibody

  • 3
  • 4
Primary Antibodies

AB239517

Anti-IL-1 alpha antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-1 alpha antibody [C1] (AB239517)

  • 0
  • 0
Primary Antibodies

AB239584

Anti-IL-1 alpha antibody [C5]

Western blot - Anti-IL-1 alpha antibody [C5] (AB239584)

  • 0
  • 0
Proteins & Peptides

AB256050

Recombinant mouse IL-1 alpha protein (Active)

Bioactive

Functional Studies - Recombinant mouse IL-1 alpha protein (Active) (AB256050)

  • 0
  • 0

組合せ製品

Assay Kits

AB178008

Human IL-1a ELISA Kit

Recombinant|SimpleStep

ELISA - Human IL-1a ELISA Kit (AB178008)

  • 0
  • 0
Primary Antibodies

AB283818

Anti-IL-1 beta antibody [RM1009]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry - Anti-IL-1 beta antibody [RM1009] (AB283818)

  • 0
  • 0
Primary Antibodies

AB215191

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal (AB215191)

  • 5
  • 4
Primary Antibodies

AB215203

Anti-cleaved N-terminal GSDMD antibody [EPR20829-408]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] (AB215203)

  • 5
  • 4
Primary Antibodies

AB215715

Anti-TGF beta 1 antibody [EPR21143]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-TGF beta 1 antibody [EPR21143] (AB215715)

  • 5
  • 7
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).
See full target information IL1A
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 19:314 PubMed38824534

2024

Long non-coding RNA MEG3 knockdown represses airway smooth muscle cells proliferation and migration via sponging miR-143-3p/FGF9 in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaying Gu,Dengfeng Zhou

Asian Pacific journal of cancer prevention : APJCP 23:3843-3849 PubMed36444597

2022

A Comparison of the Characteristics of Cytokine Storm between Lichen Planus and Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Salmeh Kalbassi,Lida Radfar,Mohammadreza Azimi,Simin Shadanpoor,Ali Ghorbani Ranjbary

NPJ breast cancer 8:30 PubMed35260569

2022

Tocilizumab overcomes chemotherapy resistance in mesenchymal stem-like breast cancer by negating autocrine IL-1A induction of IL-6.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew W Chung,Anthony J Kozielski,Wei Qian,Jianying Zhou,Ann C Anselme,Alfred A Chan,Ping-Ying Pan,Delphine J Lee,Jenny C Chang

Frontiers in pharmacology 12:673818 PubMed34093202

2021

An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Jian Shi,Zhaozheng Li,Ling Li,Rui Zhang,Yang Bai,Junmei Li,Fang Liang,Yiting Tang

Cell death & disease 12:402 PubMed33854044

2021

A small molecule binding HMGB1 inhibits caspase-11-mediated lethality in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Wang,Zhaozheng Li,Yang Bai,Rui Zhang,Ran Meng,Fangping Chen,Haichao Wang,Timothy R Billiar,Xianzhong Xiao,Ben Lu,Yiting Tang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
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