AB7632

Anti-IL-1 alpha 抗体

Name: Anti-IL-1 alpha 抗体 (ab7632) | Abcam
Brand: Abcam Limited
SKU: AB7632
Price: 56000 JPY
Availability: InStock
Rating: 3 (4 reviews)

Anti-IL-1 alpha antibody

(4 Reviews)

(40 Publications)

Rabbit Polyclonal IL-1 alpha antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 40 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL1A.

別名を表示する

IL1F1, IL1A, Interleukin-1 alpha, IL-1 alpha, Hematopoietin-1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody (AB7632)

ICC/IF

AbReview649d9c82815626b477301f07****

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody (AB7632)

Immunocytochemical analysis of RAW 264.7 (mouse macrophage) cells labeling IL-1 alpha with ab7632 at a dilution of 1/100. Cells were fixed with paraformaldehyde, permeabilized using 0.1% Triton X-100 in 2% BSA for 15 minutes, and blocked with 2% BSA for 1 hour at 4°C. Cells were incubated with ab7632 for 16 hours at 4°C in 2% BSA/PBS. IL-1 alpha was detected using an Alexa Fluor^® 488-conjugated chicken polyclonal anti-rabbit IgG (H+L) secondary antibody (green) at a dilution of 1/500. Cell membranes were counterstained with WGA (red) and nuclei were counterstained with DAPI (blue).

Dr Mahesh Shivananjappa

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse

アプリケーション

ICC/IF, Flow Cyt, WB, ELISA, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human IL1A.

P01583

特異性

This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions. The antiserum does not recognize human IL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarily intracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active when applied to cells and is thought to have a role as a functional molecule intracellularly and can be found constitutively expressed in various cell. This antibody is also useful for neutralization of human and primate IL-1a activity in bioassays. It does not neutralize the biological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbit IL-1a. It will neutralize primate IL-a. For neutralization, it is recommended to incubate the sample with a 1:100 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used for FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/5000", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/5000", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Primates": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製に関する特記事項

whole rabbit serum purified by DEAE fractionation

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems (PubMed : 26439902). After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex (PubMed : 17507369, PubMed : 2950091). Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4 (PubMed : 17507369). In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways (PubMed : 14687581). Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage (PubMed : 15679580). In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as a signal for genotoxic stress without loss of cell integrity (PubMed : 26439902).

See full target information IL1A

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文献 (40)

Recent publications for all applications. Explore the full list and refine your search

Aging 17:1026-1042 PubMed40168539

2025

Mesenchymal stem cell-specific Sirt1 overexpression prevents sarcopenia induced by 1,25-dihydroxyvitamin D deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Chen,Biqi Ren,Jing Wang,Xingchen Liu,Xiangjiao Yi,David Goltzman,Dengshun Miao

iScience 28:112041 PubMed40092614

2025

Repeated lidocaine exposure induces synaptic and cognitive impairment in aged mice by activating microglia and neurotoxic A1 astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Chen,Haiyang Wan,Yongxin Huang,Andi Chen,Xuyang Wu,Yanhua Guo,Jianjie Wei,Pinzhong Chen,Jiangdan Jiang,Xiaochun Zheng

Annals of dermatology 36:329-340 PubMed39623608

2024

Photodynamic Effects of Topical Photosensitizer, Photodithazine Using Micro-LED for Acne Bacteria Induced Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Su Min Lee,Soo-Hyun Kim,Zun Kim,Jee-Bum Lee

International journal of oral science 16:50 PubMed38956025

2024

Synthetic high-density lipoprotein (sHDL): a bioinspired nanotherapeutics for managing periapical bone inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Renan Dal-Fabbro,Minzhi Yu,Ling Mei,Hajime Sasaki,Anna Schwendeman,Marco C Bottino

Molecular neurobiology 61:10083-10096 PubMed38696064

2024

Effects of the AMPAR Antagonist, Perampanel, on Cognitive Function in Rats Exposed to Neonatal Iron Overload.

Applications

Unspecified application

Species

Unspecified reactive species

José da Silva,Lariza Oliveira de Souza,Maria Paula Arakaki Severo,Sarah Luize Camargo Rodrigues,Patrícia Molz,Patrícia Schonhofen,Alice Laschuk Herlinger,Nadja Schröder

Frontiers in immunology 15:1348043 PubMed38390337

2024

MSC exosomes attenuate sterile inflammation and necroptosis associated with TAK1-pJNK-NFKB mediated cardiomyopathy in diabetic ApoE KO mice.

Applications

Unspecified application

Species

Unspecified reactive species

Abha Banerjee,Dinender K Singla

EJNMMI research 13:103 PubMed38019303

2023

[F]FDG PET-MR characterization of aortitis in the IL1rn mouse model of giant-cell arteritis.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel Deshayes,Caroline Baugé,Pierre-Antoine Dupont,Christophe Simard,Hanan Rida,Hubert de Boysson,Alain Manrique,Achille Aouba

Allergologia et immunopathologia 51:31-39 PubMed37422777

2023

CTRP3 regulates NF-κB and TGFβ1/Smad3 pathways to alleviate airway inflammation and remodeling in asthmatic mice induced by OVA.

Applications

Unspecified application

Species

Unspecified reactive species

Hai Lin,Jinrong Yi

Medicine 101:e31152 PubMed36316862

2022

Gene locus polymorphisms and expression levels of interleukin-1 in lumbar disc disease: A MOOSE-compliant meta-analysis and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Kunxue Yang,Qianyi Xiao,Ruijun Zhang,Dihua Meng,Jiaqi Wang,Qingjun Wei,Hua Jiang

Cell death & disease 13:722 PubMed35982051

2022

Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Yuan,Kelong Ai,Menghua Xiang,Chengliang Xie,Mingyi Zhao,Ming Wu,Hongli Li,Yueren Wu,Yueqing Cao,Can Li,Yanjun Zhong,Xiaomeng Pei,Helen Ka Wai Law,Liqian Gao,Qicai Xiao,Xinyu Yang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-1 alpha 抗体

Anti-IL-1 alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-IL-1 alpha antibody (AB7632)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

出荷温度及び保存条件

製品の状態

精製度

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (40)

Abcam product promise