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リコンビナントRabMAb

Anti-IKKi/IKKe+NAK/TBK1 抗体 [EPR25258-15] - BSA and Azide free (ab289998)

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Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Flow Cytometry (Intracellular) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Immunoprecipitation - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
  • Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25258-15] to NAK/TBK1 + IKKi/IKKe - BSA and Azide free
  • Suitable for: WB, IHC-P, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free

  • 製品の詳細

    Rabbit monoclonal [EPR25258-15] to NAK/TBK1 + IKKi/IKKe - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, IP, Flow Cyt (Intra)more details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: His-tagged mouse IKKi/IKKe or NAK/TBK1 recombinant protein(aa9-315), Whole cell lysates of: HeLa, 293T, NIH/3T3, PC-12 , HCT 116, DLD-1, RAW 264.7 , 2.4G2 (rat b cell lymphoma b lymphocyte), C6, human testis and pancreas tissue lysate. IHC-P: Human testis, glioma and cerebrum. Flow cyt. Intra.: 293T IP: DLD-1 whole cell lysate

  • 特記事項

    ab289998 is a carrier free version of ab289973. 

    This antibody does not react with Mouse species for IHC, FC and IP application and not react with Rat species for IHC application.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab289998の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 34, 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 34, 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

画像

  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    All lanes : Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] (ab289973) at 1/1000 dilution

    Lane 1 : His-tagged mouse IKKi/IKKe recombinant protein(aa9-315) 10ng
    Lane 2 : His-tagged mouse NAK/TBK1 recombinant protein(aa9-315) 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 80 kDa
    Observed band size: 34, 80 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

     

  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    All lanes : Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] (ab289973) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 26 seconds


    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

    Lysates were freshly made and used immediately to minimize protein degradation. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30420664)

  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    All lanes : Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] (ab289973) at 1/1000 dilution

    Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate
    Lane 2 : DLD-1 (Human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 4 : 2.4G2 (rat b cell lymphoma b lymphocyte) whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 26 seconds


    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

  • Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Western blot - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    All lanes : Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] (ab289973) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

    Predicted band size: 80 kDa
    Observed band size: 80 kDa


    Exposure time: 103 seconds


    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    5% NFDM/TBST was used as blocking and diluting buffer and concentration. 

    Low expression tissue: pancreas(PMID:21171089).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling NAK/TBK1 + IKKi/IKKe with ab289973 at 1/500 (1.088 μg/ml), followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human cerebrum (PMID: 21171089). The section was incubated with ab289973 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human testis tissue labelling NAK/TBK1 + IKKi/IKKe with ab289973 at 1/500 (1.088 μg/ml), followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human testis. The section was incubated with ab289973 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labelling NAK/TBK1 + IKKi/IKKe with ab289973 at 1/500 (1.088 μg/ml), followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human glioma (PMID: 21171089). The section was incubated with ab289973 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed using ab289973, the same antibody clone in a different buffer formulation. 

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling NAK/TBK1 + IKKi/IKKe with ab289973 at 1/500 (1.088 μg/ml), followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human pancreas (PMID: 28069799). The section was incubated with ab289973 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: PBS was used instead of primary antibody followed by ready to use secondary antibody rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

  • Flow Cytometry (Intracellular) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Flow Cytometry (Intracellular) - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed by ab289973, the same antibody clone but in a different buffer formulation. 

    Flow cytometric analysis of 4% paraformaldehyde, 90% methanol 293T (Human embryonic kidney epithelial cell) labelling NAK/TBK1 + IKKi/IKKe with ab289973 at 1/50 dilution (Red) compared with a (ab172730) isotype control (Black)and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. 

  • Immunoprecipitation - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Immunoprecipitation - Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

    This data was developed by ab289973, the same antibody clone but in a different buffer formulation. 

    NAK/TBK1 + IKKi/IKKe was immunoprecipitated from 0.35 mg DLD-1 (Human colorectal adenocarcinoma cell line) whole cell lysate 10 μg with ab289973 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab289973 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. 

    Lane 1 (Input): DLD-1 whole cell lysate 10 μg

    Lane 2 (+): DLD-1 whole cell lysate

    Lane 3 (-):Rabbit monoclonal IgG (ab172730) instead of ab289973 in DLD-1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes. 

  • Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)
    Anti-IKKi/IKKe+NAK/TBK1 antibody [EPR25258-15] - BSA and Azide free (ab289998)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab289998 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab289998 は論文での使用が確認できていません。

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