Anti-IKK beta 抗体 (ab183966)
Key features and details
- Rabbit polyclonal to IKK beta
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IKK beta antibody
IKK beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IKK beta -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human IKK beta aa 185-448. The exact sequence is proprietary.
Database link: O14920 -
ポジティブ･コントロール
- Jurkat, Raji, K562, THP1 and NCI-H929 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/500.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
1/500.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/500. |
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IP
Use at an assay dependent concentration. |
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IHC-P
1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
ターゲット情報
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機能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. -
組織特異性
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
細胞内局在
Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 3551 Human
- Omim: 603258 Human
- SwissProt: O14920 Human
- Unigene: 597664 Human
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別名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
画像
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Western blot - Anti-IKK beta antibody (ab183966)All lanes : Anti-IKK beta antibody (ab183966) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Raji whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : THP-1 whole cell lysate
Lane 5 : NCI-H929 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 87 kDa10 % SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody (ab183966)Immunofluorescence analysis of Jurkat cells fixed in 4% paraformaldehyde at RT for 15 min stained with ab183966, dilution 1:500 in green. Blue is Hoechst 33342 staining of nuclear DNA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody (ab183966)Immunohistochemical analysis of paraffin-embedded human breast carcinoma labelling IKK beta with ab183966 at 1/500. Antigen retrieval was performed with EDTA based buffer (pH 8.0) for 15 minutes.
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Western blot - Anti-IKK beta antibody (ab183966)All lanes : Anti-IKK beta antibody (ab183966) at 1/10000 dilution
Lane 1 : Non-transfected 293T whole cell extracts
Lane 2 : Transfected (+) 293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 87 kDa -
Immunoprecipitation - Anti-IKK beta antibody (ab183966)IKK beta was immunoprecipitated from A431 whole cell extracts with 5ug of ab183966. An anti-rabbit IgG was used as a secondary reagent.
Lane 1: A431 whole cell extracts
Lane 2: anti-Rabbit IgG control instead of ab183966 in A431 whole cell extracts
Lane 3: ab183966 IP in A431 whole cell extracts
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab183966 は論文での使用が確認できていません。