Anti-IKK alpha (phospho T23) 抗体 (ab38515)
Key features and details
- Rabbit polyclonal to IKK alpha (phospho T23)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IKK alpha (phospho T23) antibody
IKK alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IKK alpha (phospho T23) -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IKK alpha aa 1-100 (phospho T23).
Database link: O15111 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38515の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. -
組織特異性
Widely expressed. -
関連疾患
Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1147 Human
- Omim: 600664 Human
- SwissProt: O15111 Human
- Unigene: 198998 Human
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別名
- chuk antibody
- CHUK1 antibody
- Conserved Helix Loop Helix Ubiquitous Kinase antibody
see all
画像
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Western blot - Anti-IKK alpha (phospho T23) antibody (ab38515)All lanes : Anti-IKK alpha (phospho T23) antibody (ab38515) at 1/500 dilution
Lane 1 : MDA-MB-435 cell extract.
Lane 2 : MDA-MB-435 cell extract + EGF ( The cells were treated with 200ng/ml EGF for 5 minutes).
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L)
Predicted band size: 85 kDa
Observed band size: 85 kDa
Lanes can be loaded with 5-30µg of total protein. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK alpha (phospho T23) antibody (ab38515)ab38515 at a 1:50 dilution staining IKK alpha in Human colon carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.Left image : untreated.
Right image : treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-IKK alpha (phospho T23) antibody (ab38515)ICC/IF image of ab38515 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38515, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab38515 は 39 報の論文で使用されています。
- Ji W et al. Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634. J Orthop Translat 36:132-144 (2022). PubMed: 36185580
- Lu H et al. TNF-α induces AQP4 overexpression in astrocytes through the NF-κB pathway causing cellular edema and apoptosis. Biosci Rep 42:N/A (2022). PubMed: 35260880
- Yang M et al. Influence of Androgen Receptor Antagonist MDV3100 Therapy on Rats With Benign Prostatic Hyperplasia. Int Neurourol J 25:219-228 (2021). PubMed: 34610715
- Chen YY et al. IκB kinase promotes Nrf2 ubiquitination and degradation by phosphorylating cylindromatosis, aggravating oxidative stress injury in obesity-related nephropathy. Mol Med 27:137 (2021). PubMed: 34711178
- Tian S et al. Curcumin inhibits the growth of liver cancer by impairing myeloid-derived suppressor cells in murine tumor tissues. Oncol Lett 21:286 (2021). PubMed: 33732362