AB24783

Anti-IKB alpha (phospho Y42) 抗体

Name: Anti-IKB alpha (phospho Y42) 抗体 (ab24783) | Abcam
Brand: Abcam Limited
SKU: AB24783
Price: 78000 JPY
Availability: InStock

Anti-IKB alpha (phospho Y42) antibody

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(24 Publications)

Rabbit Polyclonal IKB alpha phospho Y42 antibody. Suitable for WB and reacts with Human samples. Cited in 24 publications. Immunogen corresponding to Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

1 Images

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Western blot - Anti-IKB alpha (phospho Y42) antibody (AB24783)

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.

Lane 1:

Western blot - Anti-IKB alpha (phospho Y305) antibody (<a href='/products/primary-antibodies/ikb-alpha-phospho-y305-antibody-ab24784'>ab24784</a>)

Lane 1:

Western blot - Anti-IKB alpha (phospho Y42) antibody (ab24783)

Predicted band size: 35 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human NFKBIA phospho Y42 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P25963

特異性

There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.

Useful in detection of phosphorylated IKB alpha (Tyr-42).

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier.

バッファー組成

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed : 1493333, PubMed : 36651806, PubMed : 7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed : 7479976, PubMed : 7628694, PubMed : 7796813, PubMed : 7878466).

See full target information NFKBIA phospho Y42

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文献 (24)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34490475

2021

Remote ischemic post‑conditioning alleviates ischemia/reperfusion‑induced intestinal injury via the ERK signaling pathway‑mediated RAGE/HMGB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Mi,Nan Zhang,Jiyun Wan,Ming Cheng,Jianping Liao,Xiao Zheng

Molecular medicine reports 22:5313-5325 PubMed33174011

2020

Knockdown of exosome‑mediated lnc‑PVT1 alleviates lipopolysaccharide‑induced osteoarthritis progression by mediating the HMGB1/TLR4/NF‑κB pathway via miR‑93‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Meng,Siqiang Qiu,Long Sun,Jinliang Zuo

Bioengineered 11:1047-1057 PubMed32970961

2020

Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic β-cells via MAPK/NF-kb signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tangying Li,Huibiao Quan,Huachuan Zhang,Leweihua Lin,Qianying Ou,Kaining Chen

BioFactors (Oxford, England) 46:813-820 PubMed32525617

2020

LncRNA H19 aggravates TNF-α-induced inflammatory injury via TAK1 pathway in MH7A cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jialiang Yang,Yixuan Li,Lili Wang,Zhenchun Zhang,Zunzhong Li,Qian Jia

Life sciences 257:117889 PubMed32502541

2020

Taurine attenuates liver autophagy and injury of offspring in gestational diabetic mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Luo,Yue Tian,Chunrong Zhao

Life sciences 254:117742 PubMed32360619

2020

Baicalin attenuates fibrogenic process in human renal proximal tubular cells (HK-2) exposed to diabetic milieu.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Eun Nam,So Yeon Jo,Chul Woo Ahn,Yu Sik Kim

BioFactors (Oxford, England) 46:831-838 PubMed32027070

2020

MicroRNA-31 weakens cisplatin resistance of medulloblastoma cells via NF-κB and PI3K/AKT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ma,Wei Cao,Meili Ding

Cell cycle (Georgetown, Tex.) 19:207-217 PubMed31876239

2019

Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Lv,Su Zhang,Xiaohong Hao

Molecular medicine reports 21:123-130 PubMed31746402

2019

Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia‑reperfusion rats.

Applications

Unspecified application

Species

Unspecified reactive species

Cun-Xian Shi,Jin Jin,Xue-Qin Wang,Teng Song,Guang-Hong Li,Ke-Zhong Li,Jia-Hai Ma

Experimental and therapeutic medicine 18:4406-4412 PubMed31772635

2019

Chrysophanol alleviates myocardial injury in diabetic mice by regulating the SIRT1/HMGB1/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Xue,Jing Zhao,Aibin Zheng,Lin Li,Huaqin Chen,Wenjuan Tu,Ning Zhang,Zhangbin Yu,Qiuwei Wang,Meng Gu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IKB alpha (phospho Y42) 抗体

Anti-IKB alpha (phospho Y42) antibody

Western blot - Anti-IKB alpha (phospho Y42) antibody (AB24783)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (24)

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Abcam product promise