Anti-IGLL1 抗体 (ab154517)
Key features and details
- Rabbit polyclonal to IGLL1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IGLL1 antibody -
製品の詳細
Rabbit polyclonal to IGLL1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 130-213 of Human IGLL1 (Uniprot ID: P15814).
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ポジティブ･コントロール
- THP1 whole cell lysate; Human colon epithelium lymphocyte tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154517の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 10mM Citrate buffer |
ターゲット情報
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機能
Critical for B-cell development. -
組織特異性
Expressed only in pre-B-cells and a special B-cell line (which is surface Ig negative). -
関連疾患
Defects in IGLL1 are the cause of agammaglobulinemia type 2 (AGM2) [MIM:613500]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. -
配列類似性
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3543 Human
- Omim: 146770 Human
- SwissProt: P15814 Human
- Unigene: 348935 Human
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別名
- 14.1 antibody
- AGM2 antibody
- CD179 antigen-like family member B antibody
see all
画像
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Western blot - Anti-IGLL1 antibody (ab154517)Anti-IGLL1 antibody (ab154517) at 1/1000 dilution + THP1 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGLL1 antibody (ab154517)Immunohistochemical analysis of paraffin-embedded Human colon epithelium lymphocyte tissue labeling IGLL1 with ab154517 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154517 は 1 報の論文で使用されています。
- Zhang K et al. Super Enhancer Regulatory Gene FYB1 Promotes the Progression of T Cell Acute Lymphoblastic Leukemia by Activating IGLL1. J Immunol Res 2023:3804605 (2023). PubMed: 37767202