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リコンビナントRabMAb

Anti-IgG4 抗体 [EP4420] (ab109493)

Submit a review Q&A (3)References (22)
Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)
  • Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
  • Anti-IgG4 antibody [EP4420] (ab109493)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4420] to IgG4
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-IgG4 antibody [EP4420]
    IgG4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP4420] to IgG4

  • 由来種

    Rabbit

  • 特異性

    Does not cross-react with IgG1, IgG2, or IgG3.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human IgG4 recombinant protein; Human spleen lysate, Human plasma lysate IHC-P: Human tonsil tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP4420

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109493の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Predicted molecular weight: 36 kDa.

For unpurified, use at 1/1000 - 1/10000.
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Predicted molecular weight: 36 kDa.

For unpurified, use at 1/1000 - 1/10000.
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性

    IgG4 antibodies will dominate the IgG response in schistosomiasis, lymphatic filariasis, and in patients after allergen immunotherapy. Unlike the other IgG subclasses, IgG4 does not activate complement. A combined IgA-IgG4 deficiency has been associated with recurrent pyogenic infections.

  • 細胞内局在

    Secreted

  • 参照データベース

    • 別名

      • Ig gamma 4 chain C region antibody
      • IGHG4 antibody
      • immunoglobulin heavy chain constant region gamma 4 antibody
      • Immunoglobulin heavy constant gamma 4 (G4m marker) antibody
      • MGC117419 antibody
      see all

    画像

    • Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
      Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
      All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution (Purified)

      Lane 1 : Human spleen lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IgG4 antibody [EP4420] (ab109493)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling IgG4 with Purified ab109493 at 1:4000 dilution (0.023 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
      Western blot - Anti-IgG4 antibody [EP4420] (ab109493)
      All lanes : Anti-IgG4 antibody [EP4420] (ab109493) at 1/1000 dilution

      Lane 1 : IgG4 Recombinant protein at 0.005 µg
      Lane 2 : IgG1 Recombinant protein at 0.01 µg
      Lane 3 : IgG2 Recombinant protein at 0.01 µg
      Lane 4 : IgG3 Recombinant protein at 0.01 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 180 seconds.

    • Anti-IgG4 antibody [EP4420] (ab109493)
      Anti-IgG4 antibody [EP4420] (ab109493)

    参考文献 (22)

    ab109493 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109493 は 22 報の論文で使用されています。

    • Warren MC  et al. SRF617 Is a Potent Inhibitor of CD39 with Immunomodulatory and Antitumor Properties. Immunohorizons 7:366-379 (2023). PubMed: 37219538
    • Kosaka S  et al. Gastrointestinal IgG4 Deposition Is a New Histopathological Feature of Eosinophilic Gastroenteritis. Dig Dis Sci 67:3639-3648 (2022). PubMed: 34499268
    • Toney NJ  et al. Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers. J Transl Med 20:112 (2022). PubMed: 35255925
    • Chen J  et al. Esophageal Mucosal Permeability as a Surrogate Measure of Cure in Eosinophilic Esophagitis. J Clin Med 11:N/A (2022). PubMed: 35888006
    • Deng Z  et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Question

    Can you please let me know if any of the following Anti- human IgG2 primary antibodies cross react with Rhesus IgG2?    I am also interested in purchasing Anti- human IgG3 and IgG4  primary antibodies that recognize Rhesus IgG3 and IgG4, respectively.

    Read More
    Answer

    I have reviewed these products and have been in touch with the labs regarding reactivity with Rhesus as requested. We would not recommend any human IgG2 antibody which we know is directed to the hinge region, such as ab1935, against Rhesus as the homology of these is poor. However with this exception, in general there seems to be good homology between Rhesus and Human IgG subtypes. More information about human and Rhesus IgGs may be found at the links below: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782391/ http://nhpreagents.bidmc.harvard.edu/nhp/macaqueisotypes.aspx http://www.kcl.ac.uk/immunobiology/Mac_ig/ We have not tested any of these products against Rhesus. For UNTESTED applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial We could also offer enrollment in the AbTrial program for anti-IgG4 ab109493. We do not currently have any anti-IgG3 primary antibodies. More information about ab109493 can be found at the locations below: Rabbit monoclonal [EP4420] to IgG4: https://www.abcam.com/igg4-antibody-ep4420-ab109493.html

    Read More

    Question

    Buenos dias,
    Pues muchas gracias, la verdad, tenéis un servicio estupendo.
    El anticuerpo monoclonal antirabbit que podría interesarme es el siguiente: Anti-IgG4 antibody [EP4420] (ab109493).
    Gracias por todo, he quedado muy satisfecha. Es una suerte que deis esta garantía, así no da miedo arriesgarse.
    Saludos cordiales,

    Read More
    Answer

    Gracias por tu respuesta.
    Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab99466 y el envío de ab109493 como parte de la promoción para testar anticuerpos RabMab. El número de pedido es XXXXX.
    Te llegará un mail de confirmación del nuevo pedido, si necesitas información sobre el estado del mismo no dudes en contactarnos haciendo referencia al número indicado.
    Espero que este nuevo anticuerpo funcione según lo esperado. En caso contrario, te recuerdo que estos anticuerpos también tienen una garantía de 6 meses.
    Que pases un buen día.

    Read More

    Question

    Could you please tell me the expiry date for Anti-IgG4 anti body (EP4420)-(ab109493)?

    Read More
    Answer

    Thank you for contacting Abcam.

    This product is expected to remain stable for up to 1 year from receipt when stored properly at -20oC.

    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com