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リコンビナントRabMAb

Anti-IgG1 抗体 [EPR4417] (ab108969)

Submit a review Submit a question References (3)
Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
  • Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
  • OI-RD Scanning - Anti-IgG1 antibody [EPR4417] (ab108969)
  • Anti-IgG1 antibody [EPR4417] (ab108969)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4417] to IgG1
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-IgG1 antibody [EPR4417]
    IgG1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4417] to IgG1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human tonsil and human spleen lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • 解離定数（KD 値）

    KD = 3.87 x 10 -10 M
    Learn more about KD

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR4417

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108969の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

ターゲット情報

  • 関連性

    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).

  • 細胞内局在

    Secreted. Cell membrane; Single-pass membrane protein

  • 参照データベース

    • 別名

      • Ig gamma 1 chain C region antibody
      • IgG heavy chain locus antibody
      • IGHG1 antibody
      • Immunoglobulin Gm1 antibody
      • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
      see all

    画像

    • Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
      Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
      All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)

      Lane 1 : Human tonsil lysate
      Lane 2 : Human spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 52 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
      Western blot - Anti-IgG1 antibody [EPR4417] (ab108969)
      All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)

      Lane 1 : IgG1 at 0.01 µg
      Lane 2 : IgG2 at 0.01 µg
      Lane 3 : IgG3 at 0.01 µg
      Lane 4 : IgG4 at 0.01 µg
      Lane 5 : Human tonsil lysate at 10 µg
      Lane 6 : Human spleen lysate at 10 µg

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • OI-RD Scanning - Anti-IgG1 antibody [EPR4417] (ab108969)
      OI-RD Scanning - Anti-IgG1 antibody [EPR4417] (ab108969)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-IgG1 antibody [EPR4417] (ab108969)
      Anti-IgG1 antibody [EPR4417] (ab108969)

    参考文献 (3)

    ab108969 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108969 は 3 報の論文で使用されています。

    • Melani RD  et al. Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. medRxiv N/A:N/A (2021). PubMed: 34268518
    • Yang G  et al. Down-regulation of IGHG1 enhances Protoporphyrin IX accumulation and inhibits hemin biosynthesis in colorectal cancer by suppressing the MEK-FECH axis. Open Life Sci 16:930-936 (2021). PubMed: 34553073
    • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722

    レビューと Q&A

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