AB133470

Anti-IgG 抗体 [RIGG-69]

Anti-IgG antibody [RIGG-69]

20ul selling size
Recombinant
RabMAb
Lab Essentials
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(110 Publications)

Rabbit Recombinant Monoclonal IgG antibody. Suitable for WB, ELISA and reacts with Human, Mouse, Rat samples. Cited in 110 publications.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

6 Images

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Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

All lanes:

Western blot - Anti-IgG antibody [RIGG-69] (ab133470)

Lane 1:

Rat spleen tissue lysate

Lane 2:

Human tonsil tissue lysate

Predicted band size: 36 kDa

false

Lab

Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-IgG antibody [RIGG-69] (ab133470) at 1/150 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Lab

Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

purified at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human heart tissue lysate at 10 µg

Lane 3:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

Antibody dose-response curve using purified ab133470. Antigen concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

Direct ELISA antibody dose-response curve using ab133470 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

ELISA

Lab

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

Antibody dose-response curve using unpurified ab133470. Antigen concentration of 1 μg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

RIGG-69

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/200 - 1/1000.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/200 - 1/1000.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/10000", "ELISA-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "For unpurified, use 1/200 - 1/1000.", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information IgG

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文献 (110)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1636162 PubMed41019035

2025

Monoclonal immunoglobulins on red blood cells: a potential supplementary diagnostic indicator for monoclonal gammopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Benxia Bing,Xiaowei Yang,Chenghua Wang,Liang Xu,Shiqing Cheng,Yun Liu,Qiuhan Wang,Chunjuan Zhai,Bing Zhao,Rong Wang,Jing Sun

Journal of gynecologic oncology : PubMed40784723

2025

FUS-stabilized USP7 facilitates the bevacizumab resistance of ovarian cancer through deubiquitinating PTK2.

Applications

Unspecified application

Species

Unspecified reactive species

Xianping Wen,Ruocheng Xu,Ranran Li,Shuo Li,Guantai Ni

Molecular medicine (Cambridge, Mass.) 31:214 PubMed40447997

2025

Targeting casein kinase 2 and ubiquitin-specific protease 7 to modulate RUNX2-mediated osteogenesis in chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Lan,Xiao-Jun Yu,Guangsheng Ling,Yuwei Zeng,Yixi Yang,Meiyang He,Yixiao Yu,Ming Shao

Scientific reports 15:17040 PubMed40379765

2025

Experimental study of cerebral edema and expression of VEGF and AQP4 in the penumbra area of rat brain trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ai,Muhammad Usman,Hong Lu

Hereditas 162:47 PubMed40158112

2025

Activation of TRIM37 by ATF6 and degradation of ACSL4: inhibiting ferroptosis and propelling cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Li Xie,Shiying Jin,YouXiang Hou,Yina Wang

Cancers 17: PubMed40075587

2025

GluOC Induced SLC7A11 and SLC38A1 to Activate Redox Processes and Resist Ferroptosis in TNBC.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Xu,Xue Bai,Keting Dong,Qian Du,Ping Ma,Ziqian Zhang,Jianhong Yang

Molecular medicine (Cambridge, Mass.) 30:267 PubMed39716068

2024

RBM15-dependent m6A modification mediates progression of non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man Wang,Yujiao Qin,Xiaoqi Ai,Xiuhua Liu

Investigative ophthalmology & visual science 65:34 PubMed39441582

2024

ALKBH5 Regulates Corneal Neovascularization by Mediating FOXM1 M6A Demethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Hua Li,Yiyong Qian,Min Li,Manli Deng,Dexi Bi,Jun Zou

Immunity, inflammation and disease 12:e70005 PubMed39267471

2024

B7-H3 promotes nasopharyngeal carcinoma progression by regulating CD8+ T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoen Ma,Gui Chen,Hao Li,Saixuan Yang,Yali Xu,Bolin Pan,Wuping Lai,Guangui Chen,Wenjing Liao,Xiaowen Zhang

Heliyon 10:e35722 PubMed39220970

2024

ZC3H13 knockdown enhances the inhibitory effect of sevoflurane on gastric cancer cell malignancy by regulating the N6-methyladenosine modification of the lncRNA DLX6-AS1.

Applications

Unspecified application

Species

Unspecified reactive species

Chundong Liu,Zeguang Chen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG 抗体 [RIGG-69]

Anti-IgG antibody [RIGG-69]

Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

Western blot - Anti-IgG antibody [RIGG-69] (AB133470)

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

ELISA - Anti-IgG antibody [RIGG-69] (AB133470)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (110)

Abcam product promise