AB124055

Anti-IgG 抗体 [R10Z8E9]

Anti-IgG antibody [R10Z8E9]

Recombinant
Lab Essentials
詳細を見る

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(19 Publications)

Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse monoclonal antibody detecting IgG in Western Blot, ELISA. Suitable for Human.

別名を表示する

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

I-ELISA

Lab

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Lab

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Expected MW : 50 kDa.

All lanes:

Western blot - Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution

All lanes:

Human serum lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>)

Predicted band size: 36 kDa

false

Exposure time: 3min

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

R10Z8E9

アイソタイプ

IgG1

キャリアフリー

交差種

Human

アプリケーション

I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-IgG antibody [R10Z8E9] (ab124055) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), ELISA in Human samples.

What is the molecular weight of IgG?
Anti-IgG [R10Z8E9] (ab124055) specifically detects a band for IgG (UniProt: P01857) at a molecular weight of 36kDa.

Other related products
We have a range of other formats of antibody clone [R10Z8E9] also available for your convenience: ab124055, Carrier free - ab256120

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information IgG

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文献 (19)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 20:20251126 PubMed40822968

2025

SATB2 promotes humeral fracture healing in rats by activating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liantao Liu,Shuai Rong,Xiaobin Zhou,Hao Li,Kepei Zhen,Chong Zheng,Kewei Li

Clinics (Sao Paulo, Brazil) 80:100653 PubMed40273496

2025

circPOLA2 promotes proliferation, invasion, migration, and epithelial-mesenchymal transition in breast cancer via the miR-1224-5p/HMGA2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

XinYan Xu,Jie Li,RuiJuan Li,YanFang Tan,ZhiBing Lu

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Discover oncology 15:401 PubMed39225900

2024

Hsa_circYARS interacts with miR-29a-3p to up-regulate IREB2 and promote laryngeal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zizhao Guo,Yuxia Zhao,Naicai Guo,Meng Xu,Xiaolei Wang

The Kaohsiung journal of medical sciences 40:699-709 PubMed39031804

2024

circUQCRC2 promotes asthma progression in children by activating the VEGFA/NF-κB pathway by targeting miR-381-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Juan Yang,Shu-Xiang Sui,Qing-Hua Zheng,Min Wang

Discover oncology 15:165 PubMed38748048

2024

circ_0006789 promotes cervical cancer development via the miR-615-5p/HSF1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Zhou,Weiwei Song,Meisong Lu

Molecular systems biology 20:719-740 PubMed38580884

2024

Genome-wide CRISPR screens identify novel regulators of wild-type and mutant p53 stability.

Applications

Unspecified application

Species

Unspecified reactive species

YiQing Lü,Tiffany Cho,Saptaparna Mukherjee,Carmen Florencia Suarez,Nicolas S Gonzalez-Foutel,Ahmad Malik,Sebastien Martinez,Dzana Dervovic,Robin Hyunseo Oh,Ellen Langille,Khalid N Al-Zahrani,Lisa Hoeg,Zhen Yuan Lin,Ricky Tsai,Geraldine Mbamalu,Varda Rotter,Patricia Ashton-Prolla,Jason Moffat,Lucia Beatriz Chemes,Anne-Claude Gingras,Moshe Oren,Daniel Durocher,Daniel Schramek

Cartilage 16:486-494 PubMed38366389

2024

HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanchi Huang,Wenjie Pan,Huanli Bao,Xiangxiang Sun,Chao Xu,Jianbing Ma

Molecular metabolism 65:101582 PubMed36030035

2022

RIPK1 and RIPK3 regulate TNFα-induced β-cell death in concert with caspase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Contreras,Noyonika Mukherjee,Renato C S Branco,Li Lin,Meghan F Hogan,Erica P Cai,Andrew A Oberst,Steven E Kahn,Andrew T Templin

Histology and histopathology 37:899-907 PubMed35451495

2022

AADAC promotes therapeutic activity of cisplatin and imatinib against ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haijing Wang,Disong Wang,Tingting Gu,Mengjiao Zhu,Ling Cheng,Wentao Dai

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IgG 抗体 [R10Z8E9]

Anti-IgG antibody [R10Z8E9]

Indirect ELISA - Anti-IgG antibody [R10Z8E9] (AB124055)

Western blot - Anti-IgG antibody [R10Z8E9] (AB124055)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (19)

Abcam product promise