Anti-IGFBP4 抗体 (ab77350)
Key features and details
- Goat polyclonal to IGFBP4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IGFBP4 antibody
IGFBP4 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IGFBP4 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Sheep, Goat, Chicken, Cow, Pig
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免疫原
Synthetic peptide:
KGELDCHQLADSFRE
, corresponding to C terminal amino acids 244-258 of Human IGFBP4 (NP_001543.2). -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77350の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
| 特記事項 |
|---|
追加情報
Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/8000.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 374271 Chicken
- Entrez Gene: 282262 Cow
- Entrez Gene: 3487 Human
- Entrez Gene: 360622 Rat
- Entrez Gene: 443470 Sheep
- Omim: 146733 Human
- SwissProt: Q05716 Cow
- SwissProt: P22692 Human
see all -
別名
- AI875747 antibody
- BP 4 antibody
- BP4 antibody
see all
画像
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Western blot - Anti-IGFBP4 antibody (ab77350)Anti-IGFBP4 antibody (ab77350) at 1 µg/ml + Human Liver lysate in RIPA buffer at 35 µg
Predicted band size: 28 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab77350 は 3 報の論文で使用されています。
- Madani AY et al. Signal Transducer and Activator of Transcription 3 (STAT3) Suppresses STAT1/Interferon Signaling Pathway and Inflammation in Senescent Preadipocytes. Antioxidants (Basel) 10:N/A (2021). PubMed: 33672392
- Contois LW et al. Insulin-like growth factor binding protein-4 differentially inhibits growth factor-induced angiogenesis. J Biol Chem 287:1779-89 (2012). WB ; Human . PubMed: 22134921
- Gardner S et al. TGF-ß inhibits muscle differentiation by blocking autocrine signaling pathways initiated by IGF-II. Mol Endocrinol 25:128-37 (2011). WB ; Mouse . PubMed: 21106882