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Anti-IGFBP3 抗体 [RM0096-11B16] (ab86461)

Reviews (1) Submit a question
Western blot - Anti-IGFBP3 antibody [RM0096-11B16] (ab86461)

    Key features and details

    • Rat monoclonal [RM0096-11B16] to IGFBP3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG2

    製品の概要

    • 製品名

      Anti-IGFBP3 antibody [RM0096-11B16]
      IGFBP3 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [RM0096-11B16] to IGFBP3

    • 由来種

      Rat

    • 特異性

      No cross reactivity seen to other IGFBP proteins.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant full length protein corresponding to Mouse IGFBP3.
      Database link: P47878

    • 特記事項


      ab86461 was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab86461の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    1/500 - 1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa.
    特記事項
    WB
    1/500 - 1/1000. Use under non reducing condition. Predicted molecular weight: 32 kDa.

    ターゲット情報

    • 機能

      IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    • 組織特異性

      Expressed by most tissues. Present in plasma.

    • 配列類似性

      Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.

    • 発生段階

      IGFBP3 levels are higher during extrauterine life and peak during puberty.

    • ドメイン

      The thyroglobulin type-1 domain mediates interaction with HN.

    • 翻訳後修飾

      Phosphorylation sites are present in the extracelllular medium.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Acid stable subunit of the 140 K IGF complex antibody
        • Binding protein 29 antibody
        • Binding protein 53 antibody
        • BP 53 antibody
        • BP53 antibody
        • Growth hormone dependent binding protein antibody
        • IBP 3 antibody
        • IBP-3 antibody
        • IBP3 antibody
        • IBP3_HUMAN antibody
        • IGF binding protein 3 antibody
        • IGF-binding protein 3 antibody
        • IGFBP 3 antibody
        • IGFBP-3 antibody
        • IGFBP3 antibody
        • Insulin Like Growth Factor Binding Protein 3 antibody
        • Insulin-like growth factor binding protein 3 precursor antibody
        • Insulin-like growth factor-binding protein 3 antibody
        see all

      画像

      参考文献 (0)

      ab86461 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab86461 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Western blot abreview for Anti-IGFBP3 antibody [RM0096-11B16]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (brain pericytes)
      Gel Running Conditions
      Reduced Denaturing (10% gel)
      Loading amount
      20 µg
      Specification
      brain pericytes
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Aug 30 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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