Anti-IGFBP1 抗体 [EPR14471(B)] (ab180948)
![Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)](https://www.abcam.co.jp/ps/products/180/ab180948/Images/ab180948-212382-anti-igfbp1-antibody-epr14471b-western-blot.jpg "Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14471(B)] to IGFBP1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IGFBP1 antibody [EPR14471(B)]
IGFBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR14471(B)] to IGFBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HepG2 cell lysate; Human placenta and fetal liver lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR14471(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
ターゲット情報
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
翻訳後修飾
Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3484 Human
- Omim: 146730 Human
- SwissProt: P08833 Human
- Unigene: 642938 Human
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別名
- AFBP antibody
- Alpha pregnancy associated endometrial globulin antibody
- Amniotic fluid binding protein antibody
see all
画像
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Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)All lanes : Anti-IGFBP1 antibody [EPR14471(B)] (ab180948) at 1/5000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
![Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)](https://www.abcam.co.jp/ps/products/180/ab180948/Images/ab180948-212381-anti-igfbp1-antibody-epr14471b-western-blot.jpg)
Western blot - Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)Anti-IGFBP1 antibody [EPR14471(B)] (ab180948) at 1/20000 dilution + HepG2 cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
![Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)](https://www.abcam.co.jp/ps/products/180/ab180948/Images/ab180948-3-benefits-of-recombinant-antibodies.png)
Anti-IGFBP1 antibody [EPR14471(B)] (ab180948)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab180948 は 3 報の論文で使用されています。
- Cui L et al. Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axis. J Biomed Sci 29:101 (2022). PubMed: 36419076
- Xu C et al. TORC2/3-mediated DUSP1 upregulation is essential for human decidualization. Reproduction 161:573-580 (2021). PubMed: 33780908
- Gong J et al. Inhibition of FASN suppresses migration, invasion and growth in hepatoma carcinoma cells by deregulating the HIF-1a/IGFBP1 pathway. Int J Oncol 50:883-892 (2017). PubMed: 28197637