Anti-IGF2BP1/IMP1 抗体 [EPR26408-18] - BSA and Azide free (ab290751)

製品名

Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free
IGF2BP1/IMP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR26408-18] to IGF2BP1/IMP1 - BSA and Azide free
由来種

Rabbit
特異性

Please note that this antibody does not react with Rat species for WB application.

This antibody has no cross-reactivity with mouse IGF2BP2 or IGF2BP3.
アプリケーション

適用あり: WB, IHC-P, Flow Cyt (Intra), ICC/IF, mIHCmore details
適用なし: IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Mouse testis tissue lysate. Caco-2, NIH/3T3, and 293T whole cell lysates. IHC-P: Human, mouse, rat testis. ICC/IF: NIH/3T3 cells. Flow Cyt (Intra): Caco-2 and NIH/3T3 cells. mIHC-P: Human testis tissue.
特記事項
ab290751 is the carrier-free version of ab290736.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C.
バッファー

pH: 7.20
Constituent: 100% PBS
キャリア・フリー

はい
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR26408-18
アイソタイプ

IgG
研究分野

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アプリケーション	Abreviews	特記事項
WB		Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)		Use at an assay dependent concentration.
ICC/IF		Use at an assay dependent concentration.
mIHC		Use at an assay dependent concentration.

特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra) Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
mIHC Use at an assay dependent concentration.

追加情報

Is unsuitable for IP.

機能

RNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities (By similarity). Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity.
組織特異性

Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
配列類似性

Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
ドメイン

The third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
翻訳後修飾

Phosphorylated. Phosphorylation may influence mRNA translation.
細胞内局在

Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
Target information above from: UniProt accession Q9NZI8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 10642 Human
- Entrez Gene: 140486 Mouse
- Entrez Gene: 303477 Rat
- Omim: 608288 Human
- SwissProt: Q9NZI8 Human
- SwissProt: O88477 Mouse
- SwissProt: Q8CGX0 Rat
- Unigene: 144936 Human
- Unigene: 294769 Mouse
- Unigene: 485325 Mouse
- Unigene: 77572 Rat
see all
別名
- Coding region determinant-binding protein antibody
- CRD BP antibody
- CRD-BP antibody
- CRDBP antibody
- IF2B1 antibody
- IF2B1_HUMAN antibody
- IGF II mRNA binding protein 1 antibody
- IGF-II mRNA-binding protein 1 antibody
- IGF2 mRNA binding protein 1 antibody
- IGF2 mRNA-binding protein 1 antibody
- IGF2BP1 antibody
- IMP 1 antibody
- IMP-1 antibody
- IMP1 antibody
- Insulin like growth factor 2 mRNA binding protein 1 antibody
- Insulin-like growth factor 2 mRNA-binding protein 1 antibody
- VICKZ family member 1 antibody
- VICKZ1 antibody
- ZBP 1 antibody
- ZBP-1 antibody
- ZBP1 antibody
- Zip code binding protein 1 antibody
- Zip code-binding protein 1 antibody
- Zipcode binding protein 1 antibody
- Zipcode-binding protein 1 antibody
see all

Multiplex immunohistochemistry - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

Fluorescence multiplex immunohistochemical analysis of paraffin-embedded Human testis tissue.

Panel A: Merged staining of anti-IGF2BP1/IMP1 (green; Opal™690), anti-TPTE (gray; Opal™520) and anti-SAGE1 (red; Opal™570) on human testis.
Panel B: Anti-IGF2BP1/IMP1 stained on cytoplasm of spermatogonia.
Panel C: Anti-TPTE stained on spermatocytes.
Panel D: Anti-SAGE1 stained on nucleus of spermatogonia.

The section was incubated in three rounds of staining: in the order of ab290736, ab250148, and ab233388 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.
Multiplex immunohistochemistry - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

Fluorescence multiplex immunohistochemical analysis of paraffin-embedded Human testis tissue.

Panel A: Merged staining of anti-AKAP4 (magenta; Opal™690), anti-TPTE (green; Opal™520) and anti-IGF2BP1/IMP1 (red; Opal™570) on human testis.
Panel B: Anti-IGF2BP1/IMP1 stained on spermatogonia.
Panel C: Anti-TPTE stained on spermatocytes.
Panel D: Anti-AKAP4 stained on spermatids.

The section was incubated in three rounds of staining: in the order of ab238887, ab250148, and ab290736 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Counterstained with DAPI.
Western blot - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

All lanes : Anti-IGF2BP1/IMP1 antibody [EPR26408-18] (ab290736) at 1/1000 dilution

Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Blocking / Diluent buffer and concentration: 5% NFDM/TBST

Exposure time:

Lane 1-3: 10 seconds
Lane 4: 26 seconds

Negative control: U-2 OS (PMID: 26917013)
Western blot - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

All lanes : Anti-IGF2BP1/IMP1 antibody [EPR26408-18] (ab290736) at 1/1000 dilution

Lane 1 : MYC/DDK-tagged mouse IGF2BP1 full-length recombinant protein 10 ng
Lane 2 : MYC/DDK- tagged mouse IGF2BP2 full-length recombinant protein 10 ng
Lane 3 : His-tagged mouse IGF2BP3 full-length recombinant protein 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa

Exposure time: 48 seconds

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST
Western blot - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

All lanes : Anti-IGF2BP1/IMP1 antibody [EPR26408-18] (ab290736) at 1/1000 dilution

Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 63 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Blocking / Dilution buffer and concentration: 5% NFDM/TBST

The expression profile observed is consistent with that described in the literature (PMID: 26917013).
Negative control: mouse liver, mouse kidney (PMID: 26917013).
Two unidentified bands around 37 kDa were observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling IGF2BP1/IMP1 with ab290736 at 1/2000 (0.284 ug/ml) followed by a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection). Positive staining on human testis (PMID:28333300) . The section was incubated with ab290736 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling IGF2BP1/IMP1 with ab290736 at 1/2000 (0.284 ug/ml) followed by a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection). Positive staining on mouse testis. The section was incubated with ab290736 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling IGF2BP1/IMP1 with ab290736 at 1/2000 (0.284 ug/ml) followed by a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection). Positive staining on rat testis. The section was incubated with ab290736 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (BOND^TM Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized NIH/3T3 (mouse embryonic fibroblast) cells lebelling IGF2BP1/IMP1 with ab290736 at 1/50 (11.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeilized Caco-2 (human colorectal adenocarcinoma epithelial cell) cells lebelling IGF2BP1/IMP1 with ab290736 at 1/50 (11.34 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in Caco-2 cell line. Negative control: U-2 OS (PMID: 23069990). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Flow Cytometry (Intracellular) - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Flow cytometry (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling IGF2BP1/IMP1 with ab290736 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

This data was developed using ab290736, the same antibody clone in a different buffer formulation.

Flow cytometry (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U-2 OS (human bone osteosarcoma epithelial cell, Left) / Caco-2 (human colorectal adenocarcinoma epithelial cell, Right) cells labelling IGF2BP1/IMP1 with ab290736 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: U-2 OS (PMID: 26917013)
Anti-IGF2BP1/IMP1 antibody [EPR26408-18] - BSA and Azide free (ab290751)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

キャリア・フリー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

Certificate of Compliance

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