Anti-IGF2BP1/IMP1 抗体 [EPR18791] (ab184305)

製品名

Anti-IGF2BP1/IMP1 antibody [EPR18791]
IGF2BP1/IMP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR18791] to IGF2BP1/IMP1
由来種

Rabbit
特異性

This antibody shows weak cross reactivity with IGF2BP2.
アプリケーション

適用あり: WB, IHC-P, IPmore details
種交差性

交差種: Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HEK-293, HeLa and K562 whole cell lysates; Human fetal liver and fetal kidney tissue lysates, C-Myc/DDK-tagged full length Human IGF2BP1 recombinant protein. IP: K562 whole cell lysate. IHC-P: Human testis, tonsil, Hodgkin's lymphoma and lung cancer tissues.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR18791
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-IGF2BP1/IMP1 antibody [EPR18791] - BSA and Azide free (ab229700)
- Alexa Fluor® 488 Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab315421)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab184305の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IHC-P		1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

特記事項
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

機能

RNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities (By similarity). Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity.
組織特異性

Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
配列類似性

Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains.
ドメイン

The third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
翻訳後修飾

Phosphorylated. Phosphorylation may influence mRNA translation.
細胞内局在

Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
Target information above from: UniProt accession Q9NZI8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 10642 Human
- Omim: 608288 Human
- SwissProt: Q9NZI8 Human
- Unigene: 144936 Human
別名
- Coding region determinant-binding protein antibody
- CRD BP antibody
- CRD-BP antibody
- CRDBP antibody
- IF2B1 antibody
- IF2B1_HUMAN antibody
- IGF II mRNA binding protein 1 antibody
- IGF-II mRNA-binding protein 1 antibody
- IGF2 mRNA binding protein 1 antibody
- IGF2 mRNA-binding protein 1 antibody
- IGF2BP1 antibody
- IMP 1 antibody
- IMP-1 antibody
- IMP1 antibody
- Insulin like growth factor 2 mRNA binding protein 1 antibody
- Insulin-like growth factor 2 mRNA-binding protein 1 antibody
- VICKZ family member 1 antibody
- VICKZ1 antibody
- ZBP 1 antibody
- ZBP-1 antibody
- ZBP1 antibody
- Zip code binding protein 1 antibody
- Zip code-binding protein 1 antibody
- Zipcode binding protein 1 antibody
- Zipcode-binding protein 1 antibody
see all

Western blot - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

All lanes : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/1000 dilution

Lane 1 : C-Myc/DDK-tagged full length Human IGF2BP1 recombinant protein
Lane 2 : C-Myc/DDK-tagged full length Human IGF2BP2 recombinant protein
Lane 3 : N-His-tagged full length Human IGF2BP3 recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer: 5% NFDM/TBST.

This antibody shows weak cross reactivity with IGF2BP2.

ab205606 was used as a loading control at 1/1000 dilution.

ab179807 was used at 1/1000 dilution.
Western blot - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Lane 1 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/5000 dilution
Lanes 2-4 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 4 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 63 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?

Exposure times: Lane 1: 8 seconds; Lanes 2,3: 15 seconds; Lane 4: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

IGF2BP1/IMP1 was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab184305 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184305 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: K562 whole cell lysate 10 μg (Input).
Lane 2: ab184305 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184305 in K562 whole cell lysate.

Exposure time: 1 second.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Faint, lower molecular mass bands are observed in some human cell lines including K562. These could be an isoform (Isoform 2 predicted to be 48.5kDa) and degradation products, as described in the literature (PMID: 11641779).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human lung cancer (PMID: 17255263) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Sporadic cytoplasmic staining in human Hodgkin’s lymphoma (PMID: 17296566) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in germinal center of human tonsil (PMID: 17296566) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling IGF2BP1/IMP1 with ab184305 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human testis (PMID: 16049158) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

Lane 1 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/10000 dilution
Lanes 2-3 : Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305) at 1/1000 dilution

Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : BxPC-3 (human pancreas adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 63 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?

Exposure times: Lane 1: 102 seconds; Lanes 2,3: 3 minutes.

Blocking/Dilution buffer: 5% NFDM/TBST.

Faint, lower molecular mass bands are observed in some human cell lines including K562. These could be an isoform (Isoform 2 predicted to be 48.5kDa) and degradation products, as described in the literature (PMID: 11641779). The BxPC-3 and MCF7 cell lines are negative controls (PMID 26917013).
Anti-IGF2BP1/IMP1 antibody [EPR18791] (ab184305)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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ab184305 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184305 は 13 報の論文で使用されています。

Liu P et al. Methyltransferase-like 3 promotes cervical cancer metastasis by enhancing cathepsin L mRNA stability in an N6-methyladenosine-dependent manner. Cancer Sci 114:837-854 (2023). PubMed: 36382580
Ye G et al. ALKBH5 facilitates CYP1B1 mRNA degradation via m6A demethylation to alleviate MSC senescence and osteoarthritis progression. Exp Mol Med 55:1743-1756 (2023). PubMed: 37524872
Zhu Y et al. N6-methyladenosine-modified oncofetal lncRNA MIR4435-2HG contributed to stemness features of hepatocellular carcinoma cells by regulating rRNA 2'-O methylation. Cell Mol Biol Lett 28:89 (2023). PubMed: 37891494
Zhang X et al. Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma. Cell Death Dis 14:827 (2023). PubMed: 38092752
Xu W et al. m6A RNA methylation-mediated NDUFA4 promotes cell proliferation and metabolism in gastric cancer. Cell Death Dis 13:715 (2022). WB ; Human . PubMed: 35977935

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (13)

レビューと Q&A