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リコンビナントRabMAb

Anti-IGF2 抗体 [EPR12221] (ab177467)

Submit a review Q&A (2)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] (ab177467)
  • Western blot - Anti-IGF2 antibody [EPR12221] (ab177467)
  • Anti-IGF2 antibody [EPR12221] (ab177467)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12221] to IGF2
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

製品の概要

  • 製品名

    Anti-IGF2 antibody [EPR12221]
    IGF2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12221] to IGF2

  • 由来種

    Rabbit

  • 特異性

    ab177467 has been found not to cross react with mouse or rat IGF-2 in western blot testing.

  • アプリケーション

    適用あり: ICC/IF, WBmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human IGF2 aa 50-150 (internal sequence). The exact sequence is proprietary.
    Database link: P01344

  • ポジティブ･コントロール

    • Recombinant human IGF2 protein (ab9575) can be used as a positive control in WB. Human serum lysate and HepG2 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177467の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50 - 1/100.
WB
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
特記事項
ICC/IF
1/50 - 1/100.
WB
1/1000 - 1/5000. Predicted molecular weight: 20 kDa.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

  • 関連疾患

    Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.

  • 配列類似性

    Belongs to the insulin family.

  • 翻訳後修飾

    O-glycosylated with a core 1 or possibly core 8 glycan.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C11orf43 antibody
      • IGF 2 antibody
      • IGF II antibody
      • IGF-II antibody
      • IGF2 antibody
      • IGF2_HUMAN antibody
      • IGFII antibody
      • INSIGF antibody
      • insulin like growth factor 2 (somatomedin A) antibody
      • Insulin like Growth Factor 2 antibody
      • Insulin like growth factor II antibody
      • Insulin like growth factor II precursor antibody
      • Insulin like growth factor type 2 antibody
      • pp9974 antibody
      • Preptin antibody
      • putative insulin like growth factor II associated protein antibody
      • Somatomedin A antibody
      • Somatomedin-A antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] (ab177467)
      Immunocytochemistry/ Immunofluorescence - Anti-IGF2 antibody [EPR12221] (ab177467)

      Immunofluorescence analysis of HepG2 cells labeling IGF2  (green)  with ab177467 at 1/50 dilution. DAPI nuclear staining (blue).

    • Western blot - Anti-IGF2 antibody [EPR12221] (ab177467)
      Western blot - Anti-IGF2 antibody [EPR12221] (ab177467)
      Anti-IGF2 antibody [EPR12221] (ab177467) at 1/1000 dilution + Human serum lysate at 10 µg

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 20 kDa

    • Anti-IGF2 antibody [EPR12221] (ab177467)
      Anti-IGF2 antibody [EPR12221] (ab177467)

    参考文献 (3)

    ab177467 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177467 は 3 報の論文で使用されています。

    • Fei Z  et al. Krüppel-like factor 4 promotes the proliferation and osteogenic differentiation of BMSCs through SOX2/IGF2 pathway. Acta Biochim Pol 69:349-355 (2022). PubMed: 35617351
    • Xu J  et al. Exosomes from cisplatin-induced dormant cancer cells facilitate the formation of premetastatic niche in bone marrow through activating glycolysis of BMSCs. Front Oncol 12:922465 (2022). PubMed: 36568212
    • Mao P  et al. LncRNA SNHG12 regulates ox-LDL-induced endothelial cell injury by the miR-218-5p/IGF2 axis in atherosclerosis. Cell Cycle 20:1561-1577 (2021). PubMed: 34313533

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    x-reactivity with IGF1 determined?

    Read More
    Answer


    We haven't tested the cross-reactivity of ab177467 with IGF-1.
    However, based on the the immunogen sequence analysis the homology of the immunogen peptide is only 50% and no continuous sequences more than 3 aa could be found.
    It seems that there may be very little cross reactivity with IGF-1 (UniProt entry number P05019).

    Read More

    Question

    Obtaining a strong 50 kDa band and a faint 15 kDa band with ab177467. There seems to be a very faint band right below 50 kDa in the datasheet WB image, do you have any idea what this band is?

    Read More
    Answer

    The laboratory completed the blocking study for ab177467 (IGF2), and found that the blocking peptide did successfully block the 50 kDa band, indicating that this band is associated with the immunogen.

    We found the 2 attached papers, which cite that a 50 kDa complex does exist for both IGF1 and IGF2 in serum, so these complexes may be present in your mouse skin as well.

    From IGF1 paper:

    “In serum, the majority (70–80%) of the IGFs exist in a 150-kDa complex comprised of one IGF molecule, IGFBP-3, and the acid labile subunit (ALS). A smaller proportion (∼20%) of the IGFs are associated with other serum IGFBPs within a 50-kDa complex, and less than 5% of the IGFs are found in the free form of 7.5 kDa.”

    From IGF2 paper:

    ˜50kDa complex formed by IGF-II and IGFBP in the circulation.

    Read More

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