AB133542

Anti-IGF1 + IGF2 抗体 [EPR5098(2)]

Name: Anti-IGF1 + IGF2 抗体 [EPR5098(2)] (ab133542) | Abcam
Brand: Abcam Limited
SKU: AB133542
Price: 31000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-IGF1 + IGF2 antibody [EPR5098(2)]

20ul selling size
RabMAb
Recombinant
詳細を見る

(1 Review)

(10 Publications)

Rabbit Recombinant Monoclonal IGF1 antibody. Suitable for ELISA, WB and reacts with Recombinant full length protein - Human samples. Cited in 10 publications.

別名を表示する

IBP1, IGF-1, IGF1, Insulin-like growth factor 1, Insulin-like growth factor I, Mechano growth factor, Somatomedin-C, IGF-I, MGF, IGF-2, PP1446, IGF2, Insulin-like growth factor 2, Insulin-like growth factor II, Somatomedin-A, T3M-11-derived growth factor, IGF-II

4 Images

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Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

The recombinant IGF-I fragment corresponds to amino acids 49-118 of human IGF-1 with the molecular weight of 10kDa.

All lanes:

Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (ab133542) at 1/1000 dilution

All lanes:

Recombinant human IGF-I fragment at 0.005 µg

Predicted band size: 22 kDa

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ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

ELISA

Lab

ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

Primary antibody ab33542 was used at concentrations between 0.1 - 1000ng/ml. An Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1 : 2500 dilution. Antigen concentration was 1000ng/ml.

Lab

Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

Blocking and diluting buffer and concentration : 5% NFDM /TBST

Predicted band size of IGF1 and IGF2 should be 22kDa, both ab133542 and anti-His tag antibody detected dimer at 44kDa.

ab181602 was used as a GAPDH loading control at 1/1000000 dilution.

All lanes:

Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (ab133542) at 1/1000 dilution

Lane 1:

HEK-293T transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

HEK-293T transfected with a human IGF1 expression vector containing a His tag whole cell lysate at 20 µg

Lane 3:

HEK-293T transfected with a human IGF2 expression vector containing a His tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 44 kDa

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Exposure time: 3s

OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5098(2)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "Tested with recombinant protein." } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed : 21076856, PubMed : 24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV : ITGB3 and ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 19578119, PubMed : 22351760, PubMed : 23243309, PubMed : 23696648). As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via promotion of STUB1/CHIP-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity).

See full target information IGF1

追加のターゲット

IGF2

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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107200 PubMed37554462

2023

Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghao Deng,Shengwei Rong,Lu Gan,Fuhua Wang,Liangxiao Bao,Fang Cai,Zheting Liao,Yu Jin,Shuhao Feng,Zihang Feng,Yiran Wei,Ruge Chen,Yangchen Jin,Yanli Zhou,Xiaoyong Zheng,Liping Huang,Liang Zhao

Stem cells international 2022:9806887 PubMed36199627

2022

Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Denghao Huang,Jie Lei,Xingrui Li,Zhonghao Jiang,Maoxuan Luo,Yao Xiao

Bioengineered 13:14138-14158 PubMed35734851

2022

microRNA-301a-3p is a potential biomarker in venous ulcers vein and gets involved in endothelial cell dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Jingchen Du,Yu Liu,Shuhui Yang,Qingshan Wang

Bioengineered 13:1304-1319 PubMed34983307

2022

Upregulating microRNA-373-3p promotes apoptosis and inhibits metastasis of hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbin Li,Nan Wang,Yuntian Xu,Xiao Chang,Jing Ke,Jun Yin

Cell death discovery 7:372 PubMed34857740

2021

PIAS1 alleviates diabetic peripheral neuropathy through SUMOlation of PPAR-γ and miR-124-induced downregulation of EZH2/STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Zixin Hou,Ji Chen,Huan Yang,Xiaoling Hu,Fengrui Yang

Antioxidants (Basel, Switzerland) 10: PubMed34829553

2021

17β-Estradiol Abrogates Oxidative Stress and Neuroinflammation after Cortical Stab Wound Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kamran Saeed,Myeung Hoon Jo,Jun Sung Park,Sayed Ibrar Alam,Ibrahim Khan,Riaz Ahmad,Amjad Khan,Rahat Ullah,Myeong Ok Kim

Oncology letters 21:283 PubMed33732359

2021

Exosomal miR-130a-3p promotes the progression of differentiated thyroid cancer by targeting insulin-like growth factor 1.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Yin,Wencheng Kong,Sixin Zheng,Yuqiang Shan,Jian Zhang,Rongchao Ying,Hao Wu

Molecular medicine reports 23: PubMed33313952

2020

Inhibition of SNCG suppresses the proliferation of lung cancer cells induced by high glucose.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Fei,Chi Xiao,Meiying Yang,Xue Zhou,Ping Gong

OncoTargets and therapy 13:4035-4048 PubMed32494158

2020

Elevated Levels of circRUNX1 in Colorectal Cancer Promote Cell Growth and Metastasis via miR-145-5p/IGF1 Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Lei Chen,Xiang-Nan Li,Chun-Xiang Ye,Hong-Yu Chen,Zhen-Jun Wang

Biochimica et biophysica acta. General subjects 1862:567-575 PubMed29174671

2017

Human IGF-I propeptide A promotes articular chondrocyte biosynthesis and employs glycosylation-dependent heparin binding.

Applications

Unspecified application

Species

Unspecified reactive species

Shuiliang Shi,Brian J Kelly,Congrong Wang,Ken Klingler,Albert Chan,George J Eckert,Stephen B Trippel

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IGF1 + IGF2 抗体 [EPR5098(2)]

Anti-IGF1 + IGF2 antibody [EPR5098(2)]

Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

ELISA - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

Western blot - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

OI-RD Scanning - Anti-IGF1 + IGF2 antibody [EPR5098(2)] (AB133542)

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (10)

Abcam product promise