Anti-IgE 抗体 [5D4] (ab7295)
Key features and details
- Mouse monoclonal [5D4] to IgE
- Suitable for: Sandwich ELISA
- Reacts with: Human
- Isotype: IgG2a
リコンビナント抗体で、ロット間での高い再現性を実現
製品の概要
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製品名
Anti-IgE antibody [5D4]
IgE 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5D4] to IgE -
由来種
Mouse -
特異性
Specific for IgE Ce2-domain specific -
アプリケーション
適用あり: Sandwich ELISAmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.
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特記事項
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis. -
ポリ/モノ
モノクローナル -
クローン名
5D4 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use at an assay dependent concentration.
Can be used as Capture antibody with recommended pair. |
特記事項 |
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Sandwich ELISA
Use at an assay dependent concentration. Can be used as Capture antibody with recommended pair. |
ターゲット情報
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関連性
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
細胞内局在
Cell Membrane -
参照データベース
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
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別名
- Igh2 antibody
- IGHE antibody
- IGHEP1 antibody
- Immunoglobulin heavy constant epsilon antibody
画像
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Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab7295 は 1 報の論文で使用されています。
- Nguyen AT et al. DOT1L regulates dystrophin expression and is critical for cardiac function. Genes Dev 25:263-74 (2011). PubMed: 21289070