Anti-IGBP1 抗体 (ab70545)
Key features and details
- Rabbit polyclonal to IGBP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IGBP1 antibody
IGBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IGBP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide from a region between residue 300 and the C-terminus (residue 339) of human IGBP1 using the numbering given in SwissProt entry P78318
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ポジティブ･コントロール
- Whole cell lysate from Ramos, K562 and NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70545の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Associated to surface IgM-receptor; may be involved in signal transduction. May be involved in regulation of the catalytic activity of type 2A-related serine/threonine phosphatases. -
組織特異性
Ubiquitously expressed with highest levels in heart, skeletal muscle and pancreas. -
関連疾患
Defects in IGBP1 are the cause of agenesis of the corpus callosum with mental retardation-ocular coloboma-micrognathia (ACCMRCM) [MIM:300472]. -
配列類似性
Belongs to the IGBP1/TAP42 family. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3476 Human
- Entrez Gene: 18518 Mouse
- Entrez Gene: 58845 Rat
- Omim: 300139 Human
- SwissProt: P78318 Human
- SwissProt: Q61249 Mouse
- SwissProt: O08836 Rat
- Unigene: 496267 Human
see all -
別名
- Alpha 4 antibody
- B cell signal transduction molecule alpha 4 antibody
- B-cell signal transduction molecule alpha 4 antibody
see all
画像
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Western blot - Anti-IGBP1 antibody (ab70545)All lanes : Anti-IGBP1 antibody (ab70545) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Ramos cells
Lane 2 : Whole cell lysate from K562 cells
Lane 3 : Whole cell lysate from NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Immunoprecipitation - Anti-IGBP1 antibody (ab70545)1mg whole cell lysate from Ramos cells was immunoprecipitated with ab70545 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane, and probed with ab70545 at 1ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70545 は 1 報の論文で使用されています。
- Feng W et al. Profiling Novel Alternative Splicing within Multiple Tissues Provides Useful Insights into Porcine Genome Annotation. Genes (Basel) 11:N/A (2020). PubMed: 33255998